Assessment of lactase activity in humans by measurement of galactitol and galactonate in serum and urine after milk intake
| العنوان: | Assessment of lactase activity in humans by measurement of galactitol and galactonate in serum and urine after milk intake |
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| المؤلفون: | Nathalie Vionnet, Kathryn J Burton, René Badertscher, Guy Vergères, Grégory Pimentel, Linda H. Münger, Carola Freiburghaus, François P. Pralong |
| المصدر: | The American Journal of Clinical Nutrition The American journal of clinical nutrition, vol. 109, no. 2, pp. 470-477 |
| بيانات النشر: | Oxford University Press, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Adult, Male, medicine.medical_specialty, Genotype, medicine.medical_treatment, Medicine (miscellaneous), Lactose, lactase activity, Polymorphism, Single Nucleotide, Lactase activity, 03 medical and health sciences, chemistry.chemical_compound, Young Adult, 0302 clinical medicine, Blood serum, Lactose Intolerance, Internal medicine, medicine, Animals, Humans, milk digestion, Lactase, Lactose intolerance, Nutrition and Dietetics, Biomarkers/metabolism, Dairy Products/adverse effects, Digestion/genetics, Galactitol/blood, Galactitol/metabolism, Galactitol/urine, Galactose/blood, Galactose/metabolism, Galactose/urine, Lactase/deficiency, Lactase/genetics, Lactase/metabolism, Lactose/blood, Lactose/metabolism, Lactose/urine, Lactose Intolerance/genetics, Lactose Intolerance/metabolism, Liver, Milk/adverse effects, Milk/chemistry, Nutrition Assessment, Postprandial Period, Sugar Acids/blood, Sugar Acids/metabolism, Sugar Acids/urine, nutrigenetics, personalized nutrition, Galactitol, Galactose, Sugar Acids, medicine.disease, Lactase persistence, Original Research Communications, 030104 developmental biology, Postprandial, Endocrinology, Milk, chemistry, 030211 gastroenterology & hepatology, Digestion, Dairy Products, galactose metabolism, Biomarkers |
| الوصف: | Background Lactase is an enzyme that hydrolyzes lactose into glucose and galactose in the small intestine, where they are absorbed. Hypolactasia is a common condition, primarily caused by genetic programming, that leads to lactose maldigestion and, in certain cases, lactose intolerance. Galactitol and galactonate are 2 products of hepatic galactose metabolism that are candidate markers for the intake of lactose-containing foods. Objectives The primary objective of the study was to explore the changes in serum and urine metabolomes during postprandial dairy product tests through the association between lactase persistence genotype and the postprandial dynamics of lactose-derived metabolites. Methods We characterized the 6-h postprandial serum kinetics and urinary excretion of lactose, galactose, galactitol, and galactonate in 14 healthy men who had consumed a single dose of acidified milk (800 g) which contained 38.8 g lactose. Genotyping of LCT-13910 C/T (rs4988235) was performed to assess primary lactase persistence. Results There were 2 distinct postprandial responses, classified as high and low metabolite responses, observed for galactose, and its metabolites galactitol and galactonate, in serum and urine. In all but 1 subject, there was a concordance between the high metabolite responses and genetic lactase persistence and between the low metabolite responses and genetic lactase nonpersistence (accuracy 0.92), galactitol and galactonate being more discriminative than galactose. Conclusions Postprandial galactitol and galactonate after lactose overload appear to be good proxies for genetically determined lactase activity. The development of a noninvasive lactose digestion test based on the measurement of these metabolites in urine could be clinically useful. This trial was registered at clinicaltrials.gov as NCT02230345. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1938-3207 0002-9165 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34df8560632f3eb76ce087d516d799a9 http://europepmc.org/articles/PMC6367971 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....34df8560632f3eb76ce087d516d799a9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19383207 00029165 |
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