Extended Spectrum Beta-Lactamase-Producing Gram-Negative Bacteria Recovered From an Amazonian Lake Near the City of Belém, Brazil
| العنوان: | Extended Spectrum Beta-Lactamase-Producing Gram-Negative Bacteria Recovered From an Amazonian Lake Near the City of Belém, Brazil |
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| المؤلفون: | Dhara Y. Freitas, Marta Tacão, Adriana Ribeiro Carneiro Folador, Susana Araújo, Rommel Thiago Jucá Ramos, Rafael A. Baraúna, Artur Silva, Isabel Henriques, Juliana S. N. Azevedo |
| المصدر: | Frontiers in Microbiology, Vol 10 (2019) Frontiers in Microbiology |
| بيانات النشر: | Frontiers Media S.A., 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Microbiology (medical), Acinetobacter baumannii, Klebsiella, Cefotaxime, antibiotic resistance, medicine.medical_treatment, lcsh:QR1-502, Microbiology, whole genome analysis, lcsh:Microbiology, 03 medical and health sciences, Antibiotic resistance, blaCTX–M, polycyclic compounds, medicine, Escherichia coli, Original Research, 030304 developmental biology, Citrobacter, 0303 health sciences, biology, 030306 microbiology, Enterobacter, biochemical phenomena, metabolism, and nutrition, Acinetobacter, bacterial infections and mycoses, biology.organism_classification, 6. Clean water, 3. Good health, Beta-lactamase, bla CTX–M, medicine.drug |
| الوصف: | Aquatic systems have been described as antibiotic resistance reservoirs, where water may act as a vehicle for the spread of resistant bacteria and resistance genes. We evaluated the occurrence and diversity of third generation cephalosporin-resistant gram-negative bacteria in a lake in the Amazonia region. This water is used for human activities, including consumption after appropriate treatment. Eighteen samples were obtained from six sites in October 2014. Water quality parameters were generally within the legislation limits. Thirty-three bacterial isolates were identified as Escherichia (n = 7 isolates), Acinetobacter, Enterobacter, and Klebsiella (n = 5 each), Pseudomonas (n = 4), Shigella (n = 3), and Chromobacterium, Citrobacter, Leclercia, Phytobacter (1 isolate each). Twenty nine out of 33 isolates (88%) were resistant to most beta-lactams, except carbapenems, and 88% (n = 29) were resistant to antibiotics included in at least three different classes. Among the beta-lactamase genes inspected, the bla CTX-M was the most prevalent (n = 12 positive isolates), followed by bla TEM (n = 5) and bla SHV (n = 4). bla CTX-M-15 (n = 5), bla CTX-M-14 (n = 1) and bla CTX-M-2 (n = 1) variants were detected in conserved genomic contexts: bla CTX-M-15 flanked by ISEcp1 and Orf477; bla CTX-M-14 flanked by ISEcp1 and IS903; and bla CTX-M-2 associated to an ISCR element. For 4 strains the transfer of bla CTX-M was confirmed by conjugation assays. Compared with the recipient, the transconjugants showed more than 500-fold increases in the MICs of cefotaxime and 16 to 32-fold increases in the MICs of ceftazidime. Two isolates (Escherichia coli APC43A and Acinetobacter baumannii APC25) were selected for whole genome analysis. APC43A was predicted as a E. coli pathogen of the high-risk clone ST471 and serotype O154:H18. bla CTX-M-15 as well as determinants related to efflux of antibiotics, were noted in APC43A genome. A. baumannii APC25 was susceptible to carbapenems and antibiotic resistance genes detected in its genome were intrinsic determinants (e.g., bla OXA-208 and bla ADC-like). The strain was not predicted as a human pathogen and belongs to a new sequence type. Operons related to metal resistance were predicted in both genomes as well as pathogenicity and resistance islands. Results suggest a high dissemination of ESBL-producing bacteria in Lake Agua Preta which, although not presenting characteristics of a strongly impacted environment, contains multi-drug resistant pathogenic strains. |
| اللغة: | English |
| DOI: | 10.3389/fmicb.2019.00364/full |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::349b19613f0f6b93bbe8520bba605b7a https://www.frontiersin.org/article/10.3389/fmicb.2019.00364/full |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....349b19613f0f6b93bbe8520bba605b7a |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.3389/fmicb.2019.00364/full |
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