Determination of clindamycin in animal plasma by high-performance liquid chromatography combined with electrospray ionization mass spectrometry
| العنوان: | Determination of clindamycin in animal plasma by high-performance liquid chromatography combined with electrospray ionization mass spectrometry |
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| المؤلفون: | Marc Cherlet, Siska Croubels, Patrick De Backer |
| المصدر: | Ghent University Academic Bibliography |
| بيانات النشر: | Wiley, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Detection limit, Spectrometry, Mass, Electrospray Ionization, Electrospray, Chromatography, Chemistry, Clindamycin, Electrospray ionization, Reproducibility of Results, Reversed-phase chromatography, Reference Standards, Mass spectrometry, High-performance liquid chromatography, Anti-Bacterial Agents, Lincomycin, Dogs, Calibration, Animals, Sample preparation, Chromatography, High Pressure Liquid, Spectroscopy, Antibacterial agent |
| الوصف: | A method for the quantification of clindamycin in animal plasma using high-performance liquid chromatography combined with electrospray ionization mass spectrometry (LC/ESI-MS/MS) is presented. Lincomycin is used as the internal standard. The sample preparation includes a simple deproteinization step with trichloroacetic acid. Chromatographic separation is achieved on an RP-18 Hypersil column using gradient elution with 0.01 M ammonium acetate and acetonitrile as mobile phase. Good linearity was observed in the range 0-10 microg ml(-1). The limit of quantification of the method is 50 ng ml(-1) and the limit of detection is 1.3 ng ml(-1). The method was shown out to be of use for pharmacokinetic studies of clindamycin formulations in dogs. |
| تدمد: | 1096-9888 1076-5174 |
| DOI: | 10.1002/jms.344 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33ffb05b57b5db7b034f6c18a0dc8b7e https://doi.org/10.1002/jms.344 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....33ffb05b57b5db7b034f6c18a0dc8b7e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10969888 10765174 |
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| DOI: | 10.1002/jms.344 |