Structure and mode of action of a novel antibacterial peptide from the blood of Andrias davidianus
| العنوان: | Structure and mode of action of a novel antibacterial peptide from the blood of Andrias davidianus |
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| المؤلفون: | Shuai Zhang, Dejing Chen, Tianli Yue, Jinjin Pei, Wengang Jin, Wenzhuo Wang, Jingzhang Geng, Hongxia Zhang |
| المصدر: | Letters in Applied Microbiology. 69:312-317 |
| بيانات النشر: | Oxford University Press (OUP), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, Urodela, Peptide, Microbial Sensitivity Tests, 01 natural sciences, Applied Microbiology and Biotechnology, Flow cytometry, 03 medical and health sciences, chemistry.chemical_compound, 010608 biotechnology, medicine, Animals, Propidium iodide, Mode of action, chemistry.chemical_classification, 0303 health sciences, Bacteria, medicine.diagnostic_test, biology, 030306 microbiology, Circular Dichroism, Cell Membrane, biology.organism_classification, Random coil, Anti-Bacterial Agents, Membrane, chemistry, Cytoplasm, Biophysics, Peptides |
| الوصف: | Andrias davidianus is widely recognized in traditional medicine as a cure-all to treat a plethora of ailments. In a previous study, a novel antibacterial peptide named andricin B was isolated from A. davidianus blood. In this study, we investigated andricin B structure and its mode of action. Circular dichroism spectra suggested that andricin B adopts a random coil state in aqueous solution and a more rigid conformation in the presence of bacteria. Moreover propidium iodide/fluorescein diacetate double staining indicated that bacteria treated with andricin B were not immediately eliminated. Rather, there is a gradual bacterial death, followed by a sublethal stage. Scanning electronic microscope imaging indicates that andricin B might form pores on cell membranes, leading to the release of cytoplasmic contents. These results were consistent with flow cytometry analysis. Furthermore, Fourier transform infrared spectroscopy suggests that andricin B induces changes in the chemical properties in the areas surrounding these “pores” on the cell membranes. Significance and Impact of the Study The results of this study suggested the new perspectives about the mode of action of antimicrobial peptide (AMP) active against sensitive bacteria. The AMP was able to be in a random coiled state in aqueous solution but to change to a more rigid one in the presence of sensitive bacteria. Exposure to AMP might not lead to immediate death of treated bacteria, rather bacteria concentration decreased gradually flattening at a sublethal stage. These findings will help people to understand better how the AMPs activate against sensitive bacteria. |
| تدمد: | 1472-765X 0266-8254 |
| DOI: | 10.1111/lam.13219 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3372274a8b829301017f261b26c48e27 https://doi.org/10.1111/lam.13219 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3372274a8b829301017f261b26c48e27 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1472765X 02668254 |
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| DOI: | 10.1111/lam.13219 |