Effect of Pentacyclic Guanidine Alkaloids from the Sponge Monanchora pulchra on Activity of α-Glycosidases from Marine Bacteria
| العنوان: | Effect of Pentacyclic Guanidine Alkaloids from the Sponge Monanchora pulchra on Activity of α-Glycosidases from Marine Bacteria |
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| المؤلفون: | Oksana Son, Lubov Slepchenko, Liudmila Tekutyeva, Larissa A. Balabanova, Galina N. Likhatskaya, I. A. Bakunina, Larisa K. Shubina, Tatyana N. Makarieva |
| المصدر: | Marine Drugs, Vol 17, Iss 1, p 22 (2019) Marine Drugs Volume 17 Issue 1 |
| بيانات النشر: | MDPI AG, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | sponge Monanchora pulchra, Stereochemistry, GH109 α-N-acetylgalactosaminidase, Pharmaceutical Science, medicine.disease_cause, 01 natural sciences, chemistry.chemical_compound, Marine bacteriophage, Drug Discovery, medicine, monanchomycalin B, Binding site, Arenibacter latericius, Guanidine, Pharmacology, Toxicology and Pharmaceutics (miscellaneous), lcsh:QH301-705.5, biology, 010405 organic chemistry, Chemistry, Alkaloid, Active site, biology.organism_classification, 0104 chemical sciences, normonanhocidin A, 010404 medicinal & biomolecular chemistry, Sponge, GH36 α-galactosidase, slow-binding irreversible inhibitor, lcsh:Biology (General), pentacyclic guanidine alkaloids, biology.protein, Bacteria, monanhocidin A |
| الوصف: | The effect of monanchomycalin B, monanhocicidin A, and normonanhocidin A isolated from the Northwest Pacific sample of the sponge Monanchora pulchra was investigated on the activity of &alpha galactosidase from the marine &gamma proteobacterium Pseudoalteromonas sp. KMM 701 (&alpha PsGal), and &alpha N-acetylgalactosaminidase from the marine bacterium Arenibacter latericius KMM 426T (&alpha NaGa). All compounds are slow-binding irreversible inhibitors of &alpha PsGal, but have no effect on &alpha NaGa. A competitive inhibitor d-galactose protects &alpha PsGal against the inactivation. The inactivation rate (kinact) and equilibrium inhibition (Ki) constants of monanchomycalin B, monanchocidin A, and normonanchocidin A were 0.166 ± 0.029 min&minus 1 and 7.70 ± 0.62 &mu M, 0.08 ± 0.003 min&minus 1 and 15.08 ± 1.60 &mu M, 0.026 ± 0.000 min&minus 1, and 4.15 ± 0.01 &mu M, respectively. The 2D-diagrams of &alpha PsGal complexes with the guanidine alkaloids were constructed with &ldquo vessel&rdquo and &ldquo anchor&rdquo parts of the compounds. Two alkaloid binding sites on the molecule of &alpha PsGal are shown. Carboxyl groups of the catalytic residues Asp451 and Asp516 of the &alpha PsGal active site interact with amino groups of &ldquo parts of the guanidine alkaloid molecules. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1660-3397 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::32c1ec4d557600c3b263aa48c96b2193 http://www.mdpi.com/1660-3397/17/1/22 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....32c1ec4d557600c3b263aa48c96b2193 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16603397 |
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