Effect of common and experimental anti-tuberculosis treatments on Mycobacterium tuberculosis growing as biofilms
| العنوان: | Effect of common and experimental anti-tuberculosis treatments on Mycobacterium tuberculosis growing as biofilms |
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| المؤلفون: | Narisa Phummarin, William A. Denny, James P. Dalton, Brent R. Copp, Siouxsie Wiles, Simon Swift, Benedict Uy |
| المصدر: | PeerJ PeerJ, Vol 4, p e2717 (2016) |
| بيانات النشر: | PeerJ Inc., 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Tuberculosis, medicine.drug_class, 030106 microbiology, Antibiotics, lcsh:Medicine, Tryptophan biosynthesis, Microbiology, General Biochemistry, Genetics and Molecular Biology, Mycobacterium tuberculosis, 03 medical and health sciences, Anti tuberculosis, medicine, Pellicle, biology, Inoculation, General Neuroscience, Biofilm, Nitroimidazole, lcsh:R, General Medicine, Ascorbic acid, biology.organism_classification, medicine.disease, Virology, 030104 developmental biology, Infectious Diseases, TB, Fluoroanthranilic acid, Lung disease, MTB, General Agricultural and Biological Sciences, Bacteria |
| الوصف: | Much is known regarding the antibiotic susceptibility of planktonic cultures ofMycobacterium tuberculosis,the bacterium responsible for the lung disease tuberculosis (TB). As planktonically-grownM. tuberculosisare unlikely to be entirely representative of the bacterium during infection, we set out to determine how effective a range of anti-mycobacterial treatments were againstM. tuberculosisgrowing as a biofilm, a bacterial phenotype known to be more resistant to antibiotic treatment. Light levels from bioluminescently-labelledM. tuberculosisH37Rv (strain BSG001) were used as a surrogate for bacterial viability, and were monitored before and after 1 week of treatment. After treatment, biofilms were disrupted, washed and inoculated into fresh broth and plated onto solid media to rescue any surviving bacteria. We found that in this phenotypic stateM. tuberculosiswas resistant to the majority of the compounds tested. Minimum inhibitory concentrations (MICs) increased by 20-fold to greater than 1000-fold, underlying the potential of this phenotype to cause significant problems during treatment. |
| اللغة: | English |
| تدمد: | 2167-8359 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31a7413be21106d21f88341fe235629b http://europepmc.org/articles/PMC5126618 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....31a7413be21106d21f88341fe235629b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21678359 |
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