Isolation of the ATP synthase subunit 6 and sequence of the mitochondrial ATP6 gene of the yeast Candida parapsilosis
| العنوان: | Isolation of the ATP synthase subunit 6 and sequence of the mitochondrial ATP6 gene of the yeast Candida parapsilosis |
|---|---|
| المؤلفون: | Emmanuel Guélin, Martine Guérin, Jean Velours |
| المصدر: | European Journal of Biochemistry. 197:105-111 |
| بيانات النشر: | Wiley, 1991. |
| سنة النشر: | 1991 |
| مصطلحات موضوعية: | Macromolecular Substances, Protein subunit, Genes, Fungal, Molecular Sequence Data, Restriction Mapping, Saccharomyces cerevisiae, Biology, Candida parapsilosis, DNA, Mitochondrial, Biochemistry, Gamma-aminobutyric acid receptor subunit alpha-1, Sequence Homology, Nucleic Acid, Genomic library, Amino Acid Sequence, Codon, DNA, Fungal, Peptide sequence, Chromatography, High Pressure Liquid, Candida, Gene Library, Base Sequence, ATP synthase, Nucleic acid sequence, biology.organism_classification, Molecular biology, Proton-Translocating ATPases, Open reading frame, Protein Biosynthesis, biology.protein, Oligonucleotide Probes |
| الوصف: | The mitochondrially translated product called subunit 6 was extracted from the yeast Candida parapsilosis mitochondria using an organic solvent mixture and purified by reverse-phase HPLC. The partial N-terminal sequence of subunit 6 reveals a post-translational cleavage site as in Saccharomyces cerevisiae. The structural mitochondrial gene ATP6 was isolated form a mitochondrial DNA library using the oligonucleotide probe procedure. The gene and the surrounding regions were cloned into M13tg130 and M13tg131 phage vectors. The insert contained an open reading frame 738-bp encoding a 246-amino-acid polypeptide. Mature subunit 6 contains 243 amino acid residues and the predicted molecular mass is 26,511 Da. The subunit shows 52% similarity with ATP synthase subunit 6 of the yeast S. cerevisiae. Comparison between protein and DNA sequences shows that the CUN codon family codes for a leucine in C. parapsilosis mitochondria. |
| تدمد: | 1432-1033 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1991.tb15887.x |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30ed7a0cb5d89a791a72d5d4e7ef42fd https://doi.org/10.1111/j.1432-1033.1991.tb15887.x |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....30ed7a0cb5d89a791a72d5d4e7ef42fd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14321033 00142956 |
|---|---|
| DOI: | 10.1111/j.1432-1033.1991.tb15887.x |