Validation of the performance of a GMO multiplex screening assay based on microarray detection
| العنوان: | Validation of the performance of a GMO multiplex screening assay based on microarray detection |
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| المؤلفون: | Norbert Hess, Serge Leimanis, Britta Kirschneit, Klára Dallmann, Guy Van den Eede, Yves Bertheau, Nicoletta Foti, Gianni Bellocchi, Katrin Neumann, Ulrich Busch, Isabel Taverniers, José Remacle, Maria Pla, Sandrine Hamels, Hermann Broll, Myriam Sneyers, Sibylle Rösel, Esther J. Kok, Adrienn Micsinai, Sven Pecoraro, Marco Mazzara, Valérie Laval, William Moens, Guillaume Mbongolo Mbella, Rupert Hochegger, Jeroen P. van Dijk, Claudia Brünen-Nieweler, Florence Nazé, Nina Papazova, Lillian Roth, Jose Luis La Paz, Colette Audeon |
| المساهمون: | Eppendorf Array Technologies, Institut Scientifique de la Santé Publique - Louis Pasteur, Partenaires INRAE, Austrian Agency for Health and Food Safety, Bundesinstitut für Risikobewertung - Federal Institute for Risk Assessment (BfR), Biomi Ltd., Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Chemische Landes- und Staatliche Veterinäruntersuchungsamt, Research Institute for Agricultural, Fisheries and Food (ILVO), Institute for Hygiene and Environment, Services déconcentrés d'appui à la recherche Ile-de-France-Versailles-Grignon, Institut National de la Recherche Agronomique (INRA), BIOlogie et GEstion des Risques en agriculture (BIOGER), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Bavarian Health and Food Safety Authority [Oberschleißheim, Germany], Lifeprint GmbH, Wageningen University and Research Centre (WUR), European Commission, Institute for Health and Consumer Protection of the JRC, European Project: 7158,CO-EXTRA(2005) |
| المصدر: | European Food Research and Technology European Food Research and Technology, Springer Verlag (Germany), 2008, 227 (6), pp.1621-1632. ⟨10.1007/s00217-008-0886-y⟩ European Food Research and Technology, 2008, 227 (6), pp.1621-1632. ⟨10.1007/s00217-008-0886-y⟩ European Food Research and Technology 227 (2008) 6 European Food Research and Technology, 227(6), 1621-1632 |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Microarray, Computer science, RIKILT - Business Unit Veiligheid & Gezondheid, zea-mays, dna, system, computer.software_genre, maize, 01 natural sciences, Biochemistry, Industrial and Manufacturing Engineering, 0404 agricultural biotechnology, pcr, Multiplex polymerase chain reaction, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Screening method, Multiplex, Screening tool, business.industry, 010401 analytical chemistry, fungi, Screening assay, 04 agricultural and veterinary sciences, General Chemistry, 040401 food science, Zea mays, quantification, 0104 chemical sciences, Biotechnology, RIKILT - Business Unit Safety & Health, Data mining, business, computer, Control methods, Food Science |
| الوصف: | International audience; A new screening method for the detection and identification of GMO, based on the use of multiplex PCR followed by microarray, has been developed and is presented. The technology is based on the identification of quite ubiquitous GMO genetic target elements first amplified by PCR, followed by direct hybridisation of the amplicons on a predefined microarray (DualChip® GMO, Eppendorf, Germany). The validation was performed within the framework of a European project (Co-Extra, contract no 007158) and in collaboration with 12 laboratories specialised in GMO detection. The present study reports the strategy and the results of an ISO complying validation of the method carried out through an inter-laboratory study. Sets of blind samples were provided consisting of DNA reference materials covering all the elements detectable by specific probes present on the array. The GMO concentrations varied from 1% down to 0.045%. In addition, a mixture of two GMO events (0.1% RRS diluted in 100% TOPAS19/2) was incorporated in the study to test the robustness of the assay in extreme conditions. Data were processed according to ISO 5725 standard. The method was evaluated with predefined performance criteria with respect to the EC CRL method acceptance criteria. The overall method performance met the acceptance criteria; in particular, the results showed that the method is suitable for the detection of the different target elements at 0.1% concentration of GMO with a 95% accuracy rate. This collaborative trial showed that the method can be considered as fit for the purpose of screening with respect to its intra- and inter-laboratory accuracy. The results demonstrated the validity of combining multiplex PCR with array detection as provided by the DualChip® GMO (Eppendorf, Germany) for the screening of GMO. The results showed that the technology is robust, practical and suitable as a screening tool. |
| وصف الملف: | application/pdf; application/octet-stream |
| اللغة: | English |
| تدمد: | 1438-2377 1438-2385 |
| DOI: | 10.1007/s00217-008-0886-y |
| DOI: | 10.1007/s00217-008-0886-y⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f90e951b3220bd924ced60d1309b3a1 https://doi.org/10.1007/s00217-008-0886-y |
| Rights: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....2f90e951b3220bd924ced60d1309b3a1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14382377 14382385 |
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| DOI: | 10.1007/s00217-008-0886-y |