The mitochondria-targeted antioxidant MitoQ modulates oxidative stress, inflammation and leukocyte-endothelium interactions in leukocytes isolated from type 2 diabetic patients
| العنوان: | The mitochondria-targeted antioxidant MitoQ modulates oxidative stress, inflammation and leukocyte-endothelium interactions in leukocytes isolated from type 2 diabetic patients |
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| المؤلفون: | Celia Bañuls, Susana Rovira-Llopis, Arantxa Martinez de Marañon, Samuel Orden, Noelia Diaz-Morales, Victor M. Victor, Irene Escribano-Lopez, Ángeles Álvarez, Antonio Hernández-Mijares, Michael P. Murphy, Milagros Rocha |
| المصدر: | Redox Biology Redox Biology, Vol 10, Iss C, Pp 200-205 (2016) |
| بيانات النشر: | Elsevier, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Mitochondrial ROS, Male, GPX1, Antioxidant, endocrine system diseases, Ubiquinone, medicine.medical_treatment, BMI, body mass index, Clinical Biochemistry, LDL, low density lipoprotein cholesterol, Anti-Inflammatory Agents, TPP, triphenylphosphonium, 030204 cardiovascular system & hematology, Pharmacology, medicine.disease_cause, Biochemistry, MitoQ, Antioxidants, chemistry.chemical_compound, 0302 clinical medicine, Glutathione Peroxidase GPX1, IR, insulin resistance, Leukocytes, lcsh:QH301-705.5, chemistry.chemical_classification, lcsh:R5-920, Anthropometry, Chemistry, Glutathione peroxidase, Type 2 diabetes, Middle Aged, Female, medicine.symptom, lcsh:Medicine (General), Research Paper, PMN, polymorphonuclear leukocytes, hs-CRP, high-sensitive C-reactive protein, HOMA-IR, homeostasis model assessment of insulin resistance, Inflammation, T2D, type 2 diabetes, 03 medical and health sciences, Organophosphorus Compounds, medicine, DBP, diastolic blood pressure, HbA1c, glycated hemoglobin, HUVEC, human umbilical vein endothelial cells, Humans, Endothelium, Aged, Reactive oxygen species, Glutathione Peroxidase, Tumor Necrosis Factor-alpha, SBP, systolic blood pressure, Organic Chemistry, Transcription Factor RelA, nutritional and metabolic diseases, HDL, high density lipoprotein cholesterol, Oxidative Stress, 030104 developmental biology, lcsh:Biology (General), Diabetes Mellitus, Type 2, Immunology, Reactive Oxygen Species, Oxidative stress |
| الوصف: | It is not known if the mitochondria-targeted antioxidants such as mitoquinone (MitoQ) can modulate oxidative stress and leukocyte-endothelium interactions in T2D patients. We aimed to evaluate the beneficial effect of MitoQ on oxidative stress parameters and leukocyte-endothelium interactions in leukocytes of T2D patients. The study population consisted of 98 T2D patients and 71 control subjects. We assessed metabolic and anthropometric parameters, mitochondrial reactive oxygen species (ROS) production, glutathione peroxidase 1 (GPX-1), NFκB-p65, TNFα and leukocyte-endothelium interactions. Diabetic patients exhibited higher weight, BMI, waist circumference, SBP, DBP, glucose, insulin, HOMA-IR, HbA1c, triglycerides, hs-CRP and lower HDL-c with respect to controls. Mitochondrial ROS production was enhanced in T2D patients and decreased by MitoQ. The antioxidant also increased GPX-1 levels and PMN rolling velocity and decreased PMN rolling flux and PMN adhesion in T2D patients. NFκB-p65 and TNFα were augmented in T2D and were both reduced by MitoQ treatment. Our findings support that the antioxidant MitoQ has an anti-inflammatory and antioxidant action in the leukocytes of T2D patients by decreasing ROS production, leukocyte-endothelium interactions and TNFα through the action of NFκB. These data suggest that mitochondria-targeted antioxidants such as MitoQ should be investigated as a novel means of preventing cardiovascular events in T2D patients. Graphical abstract fx1 Highlights • Mitochondrial ROS production was enhanced in diabetic leukocytes and reduced by MitoQ. • MitoQ treatment increased GPX-1 levels in T2D leukocytes. • MitoQ increased PMN rolling velocity and decreased PMN rolling flux and adhesion. • NFκB-p65 and TNFα were augmented in T2D and were both reduced by MitoQ treatment. • MitoQ should be investigated as a novel means of reducing cardiovascular risk in T2D. |
| اللغة: | English |
| تدمد: | 2213-2317 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f68ff2d15946d40a1b1dd7044a497b0 http://europepmc.org/articles/PMC5094376 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2f68ff2d15946d40a1b1dd7044a497b0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22132317 |
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