Structure of the radial spoke head and insights into its role in mechanoregulation of ciliary beating
| العنوان: | Structure of the radial spoke head and insights into its role in mechanoregulation of ciliary beating |
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| المؤلفون: | Nan Zhang, Gira Bhabha, Iris Grossman-Haham, Nicolas Coudray, Ronald D. Vale, Zanlin Yu, Feng Wang |
| المصدر: | Nature structural & molecular biology Nature structural & molecular biology, vol 28, iss 1 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Axoneme, Biophysics, Chlamydomonas reinhardtii, Motility, Medical and Health Sciences, Microtubules, Ciliopathies, Article, 03 medical and health sciences, 0302 clinical medicine, Structural Biology, Microtubule, Cilia, Ciliary beating, Molecular Biology, Plant Proteins, 030304 developmental biology, 0303 health sciences, biology, Chemistry, Radial spoke head, Cilium, Cryoelectron Microscopy, Biological Sciences, biology.organism_classification, Cytoskeletal Proteins, Flagella, Chemical Sciences, Motile cilium, Locomotion, 030217 neurology & neurosurgery, Developmental Biology, Signal Transduction |
| الوصف: | Motile cilia power cell locomotion and drive extracellular fluid flow by propagating bending waves from their base to tip. The coordinated bending of cilia requires mechanoregulation by the radial spoke (RS) protein complexes and the microtubule central pair (CP). Despite their importance for ciliary motility across eukaryotes, the molecular function of the RSs is unknown. Here, we reconstituted the Chlamydomonas reinhardtii RS head that abuts the CP and determined its structure using single-particle cryo-EM to 3.1-Å resolution, revealing a flat, negatively charged surface supported by a rigid core of tightly intertwined proteins. Mutations in this core, corresponding to those involved in human ciliopathies, compromised the stability of the recombinant complex, providing a molecular basis for disease. Partially reversing the negative charge on the RS surface impaired motility in C. reinhardtii. We propose that the RS-head architecture is well-suited for mechanoregulation of ciliary beating through physical collisions with the CP. |
| وصف الملف: | application/pdf |
| تدمد: | 1545-9985 1545-9993 |
| DOI: | 10.1038/s41594-020-00519-9 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f440278b13684eba81c9438ebeaa6ab https://doi.org/10.1038/s41594-020-00519-9 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2f440278b13684eba81c9438ebeaa6ab |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15459985 15459993 |
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| DOI: | 10.1038/s41594-020-00519-9 |