A negative feedback mechanism in the insulin-regulated glucose homeostasis in Japanese flounder Paralichthys olivaceus by two ways of glucose administration

التفاصيل البيبلوغرافية
العنوان: A negative feedback mechanism in the insulin-regulated glucose homeostasis in Japanese flounder Paralichthys olivaceus by two ways of glucose administration
المؤلفون: Weiqi Xu, Mengxi Yang, Kangyu Deng, Zhixiang Gu, Dong Liu, Kangsen Mai, Benyue Guo, Dongdong Han, Wenbing Zhang
بيانات النشر: Cold Spring Harbor Laboratory, 2017.
سنة النشر: 2017
مصطلحات موضوعية: medicine.medical_specialty, biology, Glucokinase, Insulin, medicine.medical_treatment, Neuropeptide Y receptor, biology.organism_classification, Olive flounder, Orexin, Endocrinology, Gluconeogenesis, Internal medicine, medicine, Glucose homeostasis, Glycolysis
الوصف: The present study comparatively analyzed the blood glucose and insulin concentration, the temporal and spatial expression of brain-gut peptides and the key enzymes of glycolysis and gluconeogenesis in Japanese flounder by intraperitoneal (IP) injection and oral (OR) administration of glucose. Samples were collected at 0, 1, 3, 5, 7, 9, 12, 24 and 48h after IP and OR, respectively. Results showed that the hyperglycemia lasted 5 hours and 21 hours in OR and IP group, respectively. The serum insulin concentration significantly decreased (1.58±0.21mIU/L) at 3h after IP glucose. However, it significantly increased at 3h (3.37±0.34mIU/L) after OR glucose. The gene expressions of prosomatostatin, neuropeptide Y, cholecystokinin precursor and orexin precursor in the brain showed different profiles between the OR and IP group. The OR not IP administration of glucose had significant effects on the gene expressions of preprovasoactive intestinal peptide, pituitary adenylate cyclase activating polypeptide and gastrin in the intestine. When the blood glucose concentration peaked in both IP and OR group, the glucokinase expression in liver was stimulated, but the expression of fructose-1,6-bisphosphatase was depressed. In conclusion, brain-gut peptides were confirmed in the present study. And the serum insulin and the brain-gut peptides have different responses between the IP and OR administration of glucose. A negative feedback mechanism in the insulin-regulated glucose homeostasis was suggested in Japanese flounder. Furthermore, this regulation could be conducted by activating PI3k-Akt, and then lead to the pathway downstream changes in glycolysis and gluconeogenesis.
DOI: 10.1101/173948
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2dee766b2a95c0455cc35ed0bb5f24da
https://doi.org/10.1101/173948
Rights: OPEN
رقم الانضمام: edsair.doi.dedup.....2dee766b2a95c0455cc35ed0bb5f24da
قاعدة البيانات: OpenAIRE