Evaluation of introgressive hybridization among Cervidae in Japan's Kinki District via two novel genetic markers developed from public NGS data
| العنوان: | Evaluation of introgressive hybridization among Cervidae in Japan's Kinki District via two novel genetic markers developed from public NGS data |
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| المؤلفون: | Ryosuke Koda, Akira Tanave, Yuki Matsumoto, Hidetoshi B. Tamate, Toshihito Takagi, Asuka Yamashiro |
| المصدر: | Ecology and Evolution, Vol 9, Iss 10, Pp 5605-5616 (2019) Ecology and Evolution |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, Cervus nippon taiouanus, animal diseases, Population, introgression, Introgression, Zoology, next‐generation sequencing, Introduced species, Okinoshima Island, Biology, 010603 evolutionary biology, 01 natural sciences, 03 medical and health sciences, Hybrid zone, lcsh:QH540-549.5, parasitic diseases, education, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, Nature and Landscape Conservation, Original Research, 0303 health sciences, education.field_of_study, Ecology, biology.organism_classification, introduced species, Genetic marker, Backcrossing, lcsh:Ecology, Formosan sika deer, Genetic monitoring |
| الوصف: | Hybridization and backcrossing of native populations with introduced species can lead to introgression and genetic alteration. In this study, we evaluated introgression in 43 deer from a potential hybrid zone around Okinoshima Island, Kinki District, Japan. This region witnessed the migration of a hybrid population (cross between the Formosan sika deer [Cervus nippon taiouanus] and other deer species) that could potentially breed with the native Japanese sika deer (C. n. centralis). We used an existing genetic marker for the mitochondrial cytochrome b gene and two novel markers for nuclear DNA, developed using publicly available next‐generation sequencing data. We identified one mainland deer with a mitochondrial haplotype identical to that of the Formosan sika deer as well as nuclear heterozygous sequences identical to those of Formosan and Japanese sika deer. This suggests that the mainland deer is a hybrid offspring of the Okinoshima population and native deer. However, only Japanese sika deer sequences were found in the other 42 samples, indicating limited introgression. Nevertheless, hybridization pre‐ and postintroduction in the Okinoshima population could cause multispecies introgression among Japanese sika deer, negatively affecting genetic integrity. We developed a simple test based on polymerase chain reaction–restriction fragment length polymorphism to detect introgression in natural populations. Our method can accelerate genetic monitoring of Japanese sika deer in Kinki District. In conclusion, to prevent further introgression and maintain genetic integrity of Japanese sika deer, we recommend establishing fences around Okinoshima Island to limit migration, besides a continued genetic monitoring of the native deer. |
| اللغة: | English |
| تدمد: | 2045-7758 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2dd994604f96e2519664acb3ee4be5af https://doaj.org/article/037dfd47f014440eb714153c54ecb639 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2dd994604f96e2519664acb3ee4be5af |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20457758 |
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