Involvement of Phospholipase Cγ1 in Mouse Egg Activation Induced by a Truncated Form of the C-kit Tyrosine Kinase Present in Spermatozoa
| العنوان: | Involvement of Phospholipase Cγ1 in Mouse Egg Activation Induced by a Truncated Form of the C-kit Tyrosine Kinase Present in Spermatozoa |
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| المؤلفون: | Raffaele Geremia, Arturo Bevilacqua, Claudio Sette, Pellegrino Rossi |
| المصدر: | The Journal of Cell Biology |
| بيانات النشر: | Rockefeller University Press, 1998. |
| سنة النشر: | 1998 |
| مصطلحات موضوعية: | Male, truncated-c-kit, Microinjections, phospholipase-C-γ1, Recombinant Fusion Proteins, Inositol Phosphates, egg-activation, Parthenogenesis, Phospholipase C gamma, Biology, Transfection, SH2 domain, Antibodies, SH3 domain, Diglycerides, src Homology Domains, Mice, Animals, Tyrosine, Adaptor Proteins, Signal Transducing, GRB2 Adaptor Protein, Diacylglycerol kinase, Settore BIO/16, Phospholipase C, Cell Cycle, src-homology-domains, Signal Transducing, Adaptor Proteins, Proteins, COS Cells, Proto-Oncogene Proteins c-kit, Spermatozoa, Protein-Tyrosine Kinases, Isoenzymes, Oocytes, Type C Phospholipases, Articles, Cell Biology, Molecular biology, Fusion protein, Cell biology |
| الوصف: | Microinjection of a truncated form of the c-kit tyrosine kinase present in mouse spermatozoa (tr-kit) activates mouse eggs parthenogenetically, and tr-kit- induced egg activation is inhibited by preincubation with an inhibitor of phospholipase C (PLC) (Sette, C., A. Bevilacqua, A. Bianchini, F. Mangia, R. Geremia, and P. Rossi. 1997. Development [Camb.]. 124:2267-2274). Co-injection of glutathione-S-transferase (GST) fusion proteins containing the src-homology (SH) domains of the gamma1 isoform of PLC (PLCgamma1) competitively inhibits tr-kit- induced egg activation. A GST fusion protein containing the SH3 domain of PLCgamma1 inhibits egg activation as efficiently as the whole SH region, while a GST fusion protein containing the two SH2 domains is much less effective. A GST fusion protein containing the SH3 domain of the Grb2 adaptor protein does not inhibit tr-kit-induced egg activation, showing that the effect of the SH3 domain of PLCgamma1 is specific. Tr-kit-induced egg activation is also suppressed by co-injection of antibodies raised against the PLCgamma1 SH domains, but not against the PLCgamma1 COOH-terminal region. In transfected COS cells, coexpression of PLCgamma1 and tr-kit increases diacylglycerol and inositol phosphate production, and the phosphotyrosine content of PLCgamma1 with respect to cells expressing PLCgamma1 alone. These data indicate that tr-kit activates PLCgamma1, and that the SH3 domain of PLCgamma1 is essential for tr-kit-induced egg activation. |
| تدمد: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.142.4.1063 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2dd87f1b5e19a6378426f165c5defb46 https://doi.org/10.1083/jcb.142.4.1063 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2dd87f1b5e19a6378426f165c5defb46 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15408140 00219525 |
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| DOI: | 10.1083/jcb.142.4.1063 |