Peach allergen Pru p 1 content is generally low in fruit but with large variation in different varieties
| العنوان: | Peach allergen Pru p 1 content is generally low in fruit but with large variation in different varieties |
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| المؤلفون: | Huijuan Jia, Zhi-qiang Wang, Zhaowei Yang, Yifan Zhu, Yuyan Zhang, Zhisheng Ma, Zhengwen Ye, Jaap H. Akkerdaas, Pere Arús, Ronald van Ree, Mingliang Yu, Ke Cao, Zhongshan Gao, Lan Zhao, Ke-xin Gan, Hangkong Liu, Jing Jin, Xiong-wei Li |
| المساهمون: | Science and Technology Department of Zhejiang Province, Zhejiang Provincial Natural Science Foundation, Producció Vegetal, Genòmica i Biotecnologia, Experimental Immunology, AII - Inflammatory diseases, APH - Global Health, APH - Personalized Medicine, Ear, Nose and Throat |
| المصدر: | IRTA Pubpro. Open Digital Archive Institut de Recerca i Tecnologia Agroalimentàries (IRTA) Clinical and Translational Allergy, Vol 11, Iss 3, Pp n/a-n/a (2021) Clinical and translational allergy, 11(3):e12034. BioMed Central Clinical and Translational Allergy Digital.CSIC. Repositorio Institucional del CSIC instname |
| بيانات النشر: | John Wiley & Sons, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Pulmonary and Respiratory Medicine, Hypoallergenic varieties, Pru p 1, Immunology, medicine.disease_cause, Allergen, Affinity chromatography, Peach allergy, Immunology and Allergy, Medicine, biology, business.industry, Research, Fresh weight, 酶联免疫吸附测试, Hypoallergenic, Protein quantification, RC581-607, 蛋白定量, Horticulture, Polyclonal antibodies, biology.protein, ELISA, Immunologic diseases. Allergy, business, 桃过敏, 低致敏品种 |
| الوصف: | [Background]: Pru p 1 is a major allergen in peach and nectarine, and the different content in varieties may affect the degree of allergic reactions. This study aimed to quantify Pru p 1 levels in representative peach varieties and select hypoallergenic Pru p 1 varieties. [Methods]: To obtain monoclonal and polyclonal antibodies, mice and rabbits, respectively, were immunized with recombinant Pru p 1.01 and Pru p 1.02. The Pru p 1 levels in fruits from 83 representative peach varieties was quantified by sandwich enzyme‐linked immunosorbent assay (sELISA). nPru p 1 was obtained through specific monoclonal antibody affinity purification and confirmed by Western blot and mass spectrometry. The variable Pru p 1 content of selected varieties was evaluated by Western blot and the expression level of encoding Pru p 1 genes by quantitative polymerase chain reaction. [Results]: A sELISA method with monoclonal and polyclonal antibodies was built for quantifying Pru p 1 levels in peach. Pru p 1 was mainly concentrated in the peel (0.20–73.44 μg/g, fresh weight), being very low in the pulp (0.05–9.62 μg/g) and not detected in wild peach. For the 78 peach and nectarine varieties, Pru p 1 content varied widely from 0.12 to 6.45 μg/g in whole fruit. We verified that natural Pru p 1 is composed of 1.01 and 1.02 isoallergens, and the Pru p 1 expression level and Pru p 1 band intensity in the immunoblots were in agreement with protein quantity determined by ELISA for some tested varieties. In some cases, the reduced levels of Pru p 1 did not coincide with low Pru p 3 in the same variety in whole fruit, while some ancient wild peach and nectarines contained low levels of both allergens, and late‐ripening yellow flesh varieties were usually highly allergenic. [Conclusion]: Pru p 1 content is generally low in peach compared to Pru p 3. Several hypoallergenic Pru p 1 and Pru p 3 varieties, “Zi Xue Tao,” “Wu Yue Xian,” and “May Fire,” were identified, which could be useful in trials for peach allergy patients. This study was funded by The Key Project for New Agricultural Cultivar Breeding in Zhejiang Province, China (2016C02052‐5), Shanghai Science and Technology Committee Rising‐Star Program (19QB1404600). |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2045-7022 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2dbbd9d802dab48db72e70b202b397c7 http://hdl.handle.net/10261/253929 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2dbbd9d802dab48db72e70b202b397c7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20457022 |
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