Cloned Viral Protein Vaccine for Foot-and-Mouth Disease: Responses in Cattle and Swine
| العنوان: | Cloned Viral Protein Vaccine for Foot-and-Mouth Disease: Responses in Cattle and Swine |
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| المؤلفون: | Douglas M. Moore, D. O. Morgan, Daniel G. Yansura, Marvin J. Grubman, P. D. McKercher, Donald Dowbenko, Howard L. Bachrach, Dennis G. Kleid, B.H. Robertson, Barbara Small |
| المصدر: | Science. 214:1125-1129 |
| بيانات النشر: | American Association for the Advancement of Science (AAAS), 1981. |
| سنة النشر: | 1981 |
| مصطلحات موضوعية: | Transcription, Genetic, Swine, Viral protein, viruses, DNA, Recombinant, Cattle Diseases, Biology, medicine.disease_cause, Virus, Viral Proteins, Plasmid, medicine, Animals, Amino Acid Sequence, Cloning, Molecular, Neutralizing antibody, Escherichia coli, Swine Diseases, Immunity, Cellular, Vaccines, Multidisciplinary, Base Sequence, RNA, DNA Restriction Enzymes, Fusion protein, Virology, Capsid, Foot-and-Mouth Disease, Protein Biosynthesis, Antibody Formation, biology.protein, Cattle |
| الوصف: | A DNA sequence coding for the immunogenic capsid protein VP3 of foot-and-mouth disease virus A12, prepared from the virion RNA, was ligated to a plasmid designed to express a chimeric protein from the Escherichia coli tryptophan promoter-operator system. When Escherichia coli transformed with this plasmid was grown in tryptophan-depleted media, approximately 17 percent of the total cellular protein was found to be an insoluble and stable chimeric protein. The purified chimeric protein competed equally on a molar basis with VP3 for specific antibodies to foot-and-mouth disease virus. When inoculated into six cattle and two swine, this protein elicited high levels of neutralizing antibody and protection against challenge with foot-and-mouth disease virus. |
| تدمد: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.6272395 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2db1ab795b342916ed486e069009cc50 https://doi.org/10.1126/science.6272395 |
| رقم الانضمام: | edsair.doi.dedup.....2db1ab795b342916ed486e069009cc50 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10959203 00368075 |
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| DOI: | 10.1126/science.6272395 |