Calcium/calmodulin-dependent protein kinase types II and IV differentially regulate CREB-dependent gene expression
| العنوان: | Calcium/calmodulin-dependent protein kinase types II and IV differentially regulate CREB-dependent gene expression |
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| المؤلفون: | L. M. Wailes, Anthony R. Means, G. S. Mcknight, C. R. Guthrie, Xinyu Zhao, R. P. Matthews |
| المصدر: | Molecular and Cellular Biology. 14:6107-6116 |
| بيانات النشر: | Informa UK Limited, 1994. |
| سنة النشر: | 1994 |
| مصطلحات موضوعية: | Transcriptional Activation, Molecular Sequence Data, Response element, In Vitro Techniques, Biology, CREB, Cell Line, Phosphoserine, Transactivation, Ca2+/calmodulin-dependent protein kinase, Cyclic AMP, Cyclic AMP Response Element-Binding Protein, Promoter Regions, Genetic, Protein kinase A, Molecular Biology, Cells, Cultured, Regulation of gene expression, Reporter gene, Base Sequence, Cell Biology, Molecular biology, Recombinant Proteins, Cell Compartmentation, Gene Expression Regulation, Calcium-Calmodulin-Dependent Protein Kinases, biology.protein, Calcium, Calcium-Calmodulin-Dependent Protein Kinase Type 2, Oligonucleotide Probes, Calcium-Calmodulin-Dependent Protein Kinase Type 4, Research Article |
| الوصف: | Phosphorylation of CREB (cyclic AMP [cAMP]- response element [CRE]-binding protein) by cAMP-dependent protein kinase (PKA) leads to the activation of many promoters containing CREs. In neurons and other cell types, CREB phosphorylation and activation of CRE-containing promoters can occur in response to elevated intracellular Ca2+. In cultured cells that normally lack this Ca2+ responsiveness, we confer Ca(2+)-mediated activation of a CRE-containing promoter by introducing an expression vector for Ca2+/calmodulin-dependent protein kinase type IV (CaMKIV). Activation could also be mediated directly by a constitutively active form of CaMKIV which is Ca2+ independent. The CaMKIV-mediated gene induction requires the activity of CREB/ATF family members but is independent of PKA activity. In contrast, transient expression of either a constitutively active or wild-type Ca2+/calmodulin-dependent protein kinase type II (CaMKII) fails to mediate the transactivation of the same CRE-containing reporter gene. Examination of the subcellular distribution of transiently expressed CaMKIV and CaMKII reveals that only CaMKIV enters the nucleus. Our results demonstrate that CaMKIV, which is expressed in neuronal, reproductive, and lymphoid tissues, may act as a mediator of Ca(2+)-dependent gene induction. |
| تدمد: | 1098-5549 0270-7306 |
| DOI: | 10.1128/mcb.14.9.6107 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d724e82a9a8089ff168063c41c3c227 https://doi.org/10.1128/mcb.14.9.6107 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2d724e82a9a8089ff168063c41c3c227 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985549 02707306 |
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| DOI: | 10.1128/mcb.14.9.6107 |