Whole-genome sequencing of Burkholderia pseudomallei from an urban melioidosis hot spot reveals a fine-scale population structure and localised spatial clustering in the environment
| العنوان: | Whole-genome sequencing of Burkholderia pseudomallei from an urban melioidosis hot spot reveals a fine-scale population structure and localised spatial clustering in the environment |
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| المؤلفون: | Audrey Rachlin, Mariana Kleinecke, Mirjam Kaestli, Glenda Harrington, Jessica R. Webb, Bart J. Currie, Mark Mayo, Vanessa Rigas |
| المصدر: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020) |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Burkholderia pseudomallei, Melioidosis, Urban Population, 030106 microbiology, lcsh:Medicine, Spatial distribution, Article, 03 medical and health sciences, Bacterial genetics, Environmental Microbiology, Northern Territory, medicine, Cluster Analysis, Humans, lcsh:Science, Phylogeny, Soil Microbiology, Whole genome sequencing, Infectious-disease epidemiology, Genetic diversity, Multidisciplinary, Whole Genome Sequencing, Phylogenetic tree, biology, Ecology, Incidence, lcsh:R, Genetic Variation, Bayes Theorem, biochemical phenomena, metabolism, and nutrition, 15. Life on land, bacterial infections and mycoses, medicine.disease, biology.organism_classification, Phylogeography, 030104 developmental biology, Geography, bacteria, Biological dispersal, lcsh:Q, Water Microbiology, Microbial genetics |
| الوصف: | Melioidosis is a severe disease caused by the environmental bacterium Burkholderia pseudomallei that affects both humans and animals throughout northern Australia, Southeast Asia and increasingly globally. While there is a considerable degree of genetic diversity amongst isolates, B. pseudomallei has a robust global biogeographic structure and genetic populations are spatially clustered in the environment. We examined the distribution and local spread of B. pseudomallei in Darwin, Northern Territory, Australia, which has the highest recorded urban incidence of melioidosis globally. We sampled soil and land runoff throughout the city centre and performed whole-genome sequencing (WGS) on B. pseudomallei isolates. By combining phylogenetic analyses, Bayesian clustering and spatial hot spot analysis our results demonstrate that some sequence types (STs) are widespread in the urban Darwin environment, while others are highly spatially clustered over a small geographic scale. This clustering matches the spatial distribution of clinical cases for one ST. Results also demonstrate a greater overall isolate diversity recovered from drains compared to park soils, further supporting the role drains may play in dispersal of B. pseudomallei STs in the environment. Collectively, knowledge gained from this study will allow for better understanding of B. pseudomallei phylogeography and melioidosis source attribution, particularly on a local level. |
| تدمد: | 2045-2322 |
| DOI: | 10.1038/s41598-020-62300-8 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d36db22553fb466aa986d742455cc0d https://doi.org/10.1038/s41598-020-62300-8 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2d36db22553fb466aa986d742455cc0d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-020-62300-8 |