Genome-Wide Transcriptional Response to Varying RpoS Levels in Escherichia coli K-12
| العنوان: | Genome-Wide Transcriptional Response to Varying RpoS Levels in Escherichia coli K-12 |
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| المؤلفون: | Joseph T. Wade, Moira Dillon, Carla J. Becker, Ciaran Evans, Lauren M. Shull, Johanna Hardin, Richard P. Bonocora, Anna J. Lee Fong, Lakshmi E. Batachari, Daniel M. Stoebel, Eliot C. Bush, Alicia N. Schep, Garrett T. Wong, Suzannah M. Beeler |
| المصدر: | Journal of Bacteriology. 199 |
| بيانات النشر: | American Society for Microbiology, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Blotting, Western, genetic processes, 030106 microbiology, Sigma Factor, Biology, medicine.disease_cause, Microbiology, Transcriptome, 03 medical and health sciences, Bacterial Proteins, Sigma factor, Transcriptional regulation, medicine, Promoter Regions, Genetic, Molecular Biology, Escherichia coli, Gene, Escherichia coli K12, Promoter, Gene Expression Regulation, Bacterial, biochemical phenomena, metabolism, and nutrition, equipment and supplies, Cell biology, carbohydrates (lipids), Regulon, Mutation, bacteria, rpoS, Research Article, Genome-Wide Association Study |
| الوصف: | The alternative sigma factor RpoS is a central regulator of many stress responses in Escherichia coli . The level of functional RpoS differs depending on the stress. The effect of these differing concentrations of RpoS on global transcriptional responses remains unclear. We investigated the effect of RpoS concentration on the transcriptome during stationary phase in rich media. We found that 23% of genes in the E. coli genome are regulated by RpoS, and we identified many RpoS-transcribed genes and promoters. We observed three distinct classes of response to RpoS by genes in the regulon: genes whose expression changes linearly with increasing RpoS level, genes whose expression changes dramatically with the production of only a little RpoS (“sensitive” genes), and genes whose expression changes very little with the production of a little RpoS (“insensitive”). We show that sequences outside the core promoter region determine whether an RpoS-regulated gene is sensitive or insensitive. Moreover, we show that sensitive and insensitive genes are enriched for specific functional classes and that the sensitivity of a gene to RpoS corresponds to the timing of induction as cells enter stationary phase. Thus, promoter sensitivity to RpoS is a mechanism to coordinate specific cellular processes with growth phase and may also contribute to the diversity of stress responses directed by RpoS. IMPORTANCE The sigma factor RpoS is a global regulator that controls the response to many stresses in Escherichia coli . Different stresses result in different levels of RpoS production, but the consequences of this variation are unknown. We describe how changing the level of RpoS does not influence all RpoS-regulated genes equally. The cause of this variation is likely the action of transcription factors that bind the promoters of the genes. We show that the sensitivity of a gene to RpoS levels explains the timing of expression as cells enter stationary phase and that genes with different RpoS sensitivities are enriched for specific functional groups. Thus, promoter sensitivity to RpoS is a mechanism that coordinates specific cellular processes in response to stresses. |
| وصف الملف: | application/pdf; application/vnd.ms-excel |
| تدمد: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.00755-16 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c7d083fe766f8e4e8ec6728c1d30963 https://doi.org/10.1128/jb.00755-16 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2c7d083fe766f8e4e8ec6728c1d30963 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985530 00219193 |
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| DOI: | 10.1128/jb.00755-16 |