Interaction of a Model Peptide on Gram Negative and Gram Positive Bacterial Sliding Clamps
| العنوان: | Interaction of a Model Peptide on Gram Negative and Gram Positive Bacterial Sliding Clamps |
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| المؤلفون: | Vincent Olieric, Bernard Lorber, Jérôme Wagner, Dominique Burnouf, Annick Dejaegere, Gilles Guichard, Philippe Dumas, Marie Landolfo, C. Andre, Isabelle Martiel, Cyrielle Da Veiga, Philippe Wolff |
| المساهمون: | Univ Bordeaux, CNRS UMR 5248, Inst Chim & Biol Membranes & Nanoobjets, INP Bordeaux, Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux (Bordeaux INP)-Centre National de la Recherche Scientifique (CNRS), GUICHARD, Gilles, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), The Swiss Light Source (SLS) (SLS-PSI), Paul Scherrer Institute (PSI), Architecture et Réactivité de l'ARN (ARN), Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre Interdisciplinaire de Nanoscience de Marseille (CINaM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Immunologie et chimie thérapeutiques (ICT), Cancéropôle du Grand Est-Centre National de la Recherche Scientifique (CNRS), Biotechnologie et signalisation cellulaire (BSC), Université de Strasbourg (UNISTRA)-Institut de recherche de l'Ecole de biotechnologie de Strasbourg (IREBS)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche de l'Ecole de biotechnologie de Strasbourg (IREBS) |
| المصدر: | ACS Infectious Diseases ACS Infectious Diseases, American Chemical Society, 2019, 5 (6), pp.1022-1034 ACS Infectious Diseases, 2019, 5 (6), pp.1022-1034. ⟨10.1021/acsinfecdis.9b00089⟩ ACS Infectious Diseases, American Chemical Society, 2019, 5 (6), pp.1022-1034. ⟨10.1021/acsinfecdis.9b00089⟩ |
| بيانات النشر: | HAL CCSD, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Models, Molecular, DNA polymerase, Protein Conformation, [SDV.BBM.BP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, Peptide, DNA-Directed DNA Polymerase, 010402 general chemistry, Crystallography, X-Ray, Gram-Positive Bacteria, Ligands, Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire, 01 natural sciences, Microbiology, 03 medical and health sciences, Drug Development, Escherichia coli, kinITC, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Antibacterial drug, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], 030304 developmental biology, Gram, Nucleic Acid Synthesis Inhibitors, sliding clamp, chemistry.chemical_classification, new antibacterials development, 0303 health sciences, [CHIM.MATE] Chemical Sciences/Material chemistry, DNA clamp, biology, [CHIM.MATE]Chemical Sciences/Material chemistry, ITC, Sciences du Vivant [q-bio]/Microbiologie et Parasitologie, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, 0104 chemical sciences, Anti-Bacterial Agents, [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, Infectious Diseases, chemistry, Mutation, biology.protein, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Peptides, ligand−target interaction, Protein Binding |
| الوصف: | Bacterial sliding clamps control the access of DNA polymerases to the replication fork and are appealing targets for antibacterial drugs development. It is therefore essential to decipher the polymerase-clamp binding mode across various bacterial species. Here, two residues of the E. coli clamp binding pocket, EcS346 and EcM362, and their cognate residues in M. tuberculosis and B. subtilis clamps, were mutated. The effects of these mutations on the interaction of a model peptide with these variant clamps were evaluated by thermodynamic, molecular dynamics, X-rays crystallography and biochemical analyses. EcM362 and corresponding residues in Gram positive clamps occupy a strategic position where a mobile residue is essential for an efficient peptide interaction. EcS346 has a more subtle function that modulates the pocket folding dynamics, while the equivalent residue in B. subtilis is essential for polymerase activity and might therefore be a Gram positive specific molecular marker. Finally, the peptide binds through an induced-fit process to Gram negative and positive pockets but the complex stability varies according to a pocket specific network of interactions. The following values have no corresponding Zotero field:auth-address: 1 Institut Européen de Chimie et Biologie , Université de Bordeaux-CNRS UMR 5248, CBMN , 2, rue Robert Escarpit , 33607 Pessac , France. 2 Swiss Light Source (SLS) , Paul-Scherrer-Institute (PSI) , 5232 Villigen , Switzerland. 3 Université de Strasbourg , CNRS, Architecture et Réactivité de l'ARN, UPR 9002, Institut de Biologie Moléculaire et Cellulaire du CNRS , 15 rue René Descartes , F-67000 Strasbourg , France. 4 Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC) , Département de Biologie Structurale et Génomique , 1 rue Laurent Fries , BP10142, 67404 Illkirch , France. 5 Biologie et Signalisation Cellulaire, ESBS , UMR7242 CNRS/Université de Strasbourg , Bld S. Brant , 67412 Illkirch Cedex , France.accession-num: 30912430 |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2373-8227 |
| DOI: | 10.1021/acsinfecdis.9b00089⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c230a773272bda13eef9e9bd64a716d https://hal.archives-ouvertes.fr/hal-03182055 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2c230a773272bda13eef9e9bd64a716d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23738227 |
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| DOI: | 10.1021/acsinfecdis.9b00089⟩ |