Characterization of the Substance P (NK-1) Receptor in Tunicamycin-Treated Transfected Cells Using a Photoaffinity Analogue of Substance P
| العنوان: | Characterization of the Substance P (NK-1) Receptor in Tunicamycin-Treated Transfected Cells Using a Photoaffinity Analogue of Substance P |
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| المؤلفون: | R. Kage, James E. Krause, Norman D. Boyd, Andrew D. Hershey, Susan E. Leeman |
| المصدر: | Journal of Neurochemistry. 64:316-321 |
| بيانات النشر: | Wiley, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | medicine.medical_specialty, Glycosylation, Light, Neurokinin A, Oligosaccharides, Substance P, CHO Cells, Transfection, Substance K, Biochemistry, Cellular and Molecular Neuroscience, chemistry.chemical_compound, Cricetinae, Internal medicine, medicine, Animals, Receptor, IC50, Tunicamycin, Chinese hamster ovary cell, Receptors, Neurokinin-1, Molecular biology, carbohydrates (lipids), Endocrinology, chemistry, Electrophoresis, Polyacrylamide Gel |
| الوصف: | Chinese hamster ovary cells expressing the N-glycosylated substance P (NK-1) receptor were treated with the glycosylation inhibitor tunicamycin and photolabeled with 125I-Bolton-Hunter-p-benzoyl-L-phenylalanine8-substance P. Two radioactive proteins of M(r) 80,000 and 46,000, representing the glycosylated and nonglycosylated substance P (NK-1) receptor, respectively, were observed. The IC50 for the inhibition of photolabeling of both receptor forms was 0.3 +/- 0.1 nM for substance P and 30 +/- 5 nM for neurokinin A (substance K). Thus, glycosylation of the substance P (NK-1) receptor has no detectable effect on the affinity of the substance P (NK-1) receptor for substance P or neurokinin A (substance K). |
| تدمد: | 1471-4159 0022-3042 |
| DOI: | 10.1046/j.1471-4159.1995.64010316.x |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b84beff784c5fc16fba30196e7ac2df https://doi.org/10.1046/j.1471-4159.1995.64010316.x |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....2b84beff784c5fc16fba30196e7ac2df |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14714159 00223042 |
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| DOI: | 10.1046/j.1471-4159.1995.64010316.x |