Regulation of skeletal muscle lipolysis and oxidative metabolism by the co-lipase CGI-58
| العنوان: | Regulation of skeletal muscle lipolysis and oxidative metabolism by the co-lipase CGI-58 |
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| المؤلفون: | Cedric Moro, Steven R. Smith, Camille Loubière, Aline Mairal, Dominique Langin, Virginie Bourlier, Pierre-Marie Badin, Katie Louche, Arild C. Rustan, Geneviève Tavernier, Maarten Coonen |
| المساهمون: | Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Pharmaceutical Biosciences, University of Oslo (UiO), Translational Research Institute for Metabolism and Diabetes and the Burnham Institute, Florida Hospital, Simon, Marie Francoise |
| المصدر: | Journal of Lipid Research, Vol 53, Iss 5, Pp 839-848 (2012) Journal of Lipid Research Journal of Lipid Research, American Society for Biochemistry and Molecular Biology, 2012, 53 (5), pp.839-48. ⟨10.1194/jlr.M019182⟩ Journal of Lipid Research, 2012, 53 (5), pp.839-48. ⟨10.1194/jlr.M019182⟩ |
| بيانات النشر: | Elsevier, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | MESH: Oxidation-Reduction, Hydrolases, Muscle Fibers, Skeletal, Peroxisome proliferator-activated receptor, Mitochondrion, Biochemistry, MESH: Lipase, Mice, 0302 clinical medicine, Endocrinology, substrate oxidation, MESH: Animals, PPAR delta, Research Articles, Cells, Cultured, chemistry.chemical_classification, [SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, 0303 health sciences, MESH: Muscle, Skeletal, MESH: Muscle Fibers, Skeletal, Myogenesis, MESH: Gene Expression Regulation, Enzymologic, Fatty Acids, MESH: Energy Metabolism, 1-Acylglycerol-3-Phosphate O-Acyltransferase, [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, humanities, MESH: Hydrolases, MESH: Fatty Acids, MESH: Glucose, mitochondria, medicine.anatomical_structure, MESH: Young Adult, Gene Knockdown Techniques, Peroxisome proliferator-activated receptor delta, Oxidation-Reduction, MESH: Cells, Cultured, MESH: Triglycerides, Adolescent, MESH: 1-Acylglycerol-3-Phosphate O-Acyltransferase, MESH: Mitochondria, Lipolysis, 030209 endocrinology & metabolism, Oxidative phosphorylation, QD415-436, Biology, Gene Expression Regulation, Enzymologic, Young Adult, 03 medical and health sciences, health services administration, mental disorders, medicine, Animals, Humans, MESH: Lipolysis, MESH: PPAR delta, Muscle, Skeletal, Glycogen synthase, MESH: Mice, Triglycerides, 030304 developmental biology, MESH: Adolescent, MESH: Humans, comparative gene identification 58, peroxisome proliferator-activated receptor, intramyocellular triacylglycerol, Skeletal muscle, Lipase, Cell Biology, MESH: Gene Knockdown Techniques, Glucose, chemistry, biology.protein, fatty acid, Energy Metabolism |
| الوصف: | International audience; We investigated here the specific role of CGI-58 in the regulation of energy metabolism in skeletal muscle. We first examined CGI-58 protein expression in various muscle types in mice, and next modulated CGI-58 expression during overexpression and knockdown studies in human primary myotubes and evaluated the consequences on oxidative metabolism. We observed a preferential expression of CGI-58 in oxidative muscles in mice consistent with triacylglycerol hydrolase activity. We next showed by pulse-chase that CGI-58 overexpression increased by more than 2-fold the rate of triacylglycerol (TAG) hydrolysis, as well as TAG-derived fatty acid (FA) release and oxidation. Oppositely, CGI-58 silencing reduced TAG hydrolysis and TAG-derived FA release and oxidation (-77%, P < 0.001), whereas it increased glucose oxidation and glycogen synthesis. Interestingly, modulations of CGI-58 expression and FA release are reflected by changes in pyruvate dehydrogenase kinase 4 gene expression. This regulation involves the activation of the peroxisome proliferator activating receptor-δ (PPARδ) by lipolysis products. Altogether, these data reveal that CGI-58 plays a limiting role in the control of oxidative metabolism by modulating FA availability and the expression of PPARδ-target genes, and highlight an important metabolic function of CGI-58 in skeletal muscle. |
| اللغة: | English |
| تدمد: | 0022-2275 |
| DOI: | 10.1194/jlr.M019182⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b84b6c759799779e42b30038b9e83fd http://www.sciencedirect.com/science/article/pii/S0022227520392142 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2b84b6c759799779e42b30038b9e83fd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00222275 |
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| DOI: | 10.1194/jlr.M019182⟩ |