Diverse p53 gene aberration in hepatocellular carcinoma detected by dual-color fluorescence in situ hybridization
| العنوان: | Diverse p53 gene aberration in hepatocellular carcinoma detected by dual-color fluorescence in situ hybridization |
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| المؤلفون: | Tsutomu Chiba, Yoshio Yamaoka, Moritoshi Kinoshita, Iwao Ikai, Akira Katsurada, Satoshi Takami, Chie Kawasome, Masahiko Kondo, Hiroyuki Marusawa, Yoshihide Ueda |
| المصدر: | Journal of Gastroenterology and Hepatology. 19:1066-1073 |
| بيانات النشر: | Wiley, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Adult, Male, Carcinoma, Hepatocellular, Loss of Heterozygosity, Biology, medicine.disease_cause, Loss of heterozygosity, Centromere, medicine, Humans, neoplasms, Gene, In Situ Hybridization, Fluorescence, Aged, Chromosome Aberrations, Analysis of Variance, Polymorphism, Genetic, Hepatology, medicine.diagnostic_test, Liver Neoplasms, Gastroenterology, Middle Aged, Genes, p53, medicine.disease, Molecular biology, digestive system diseases, Chromosome 17 (human), Case-Control Studies, Hepatocellular carcinoma, Cancer research, Female, Restriction fragment length polymorphism, Carcinogenesis, Polymorphism, Restriction Fragment Length, Chromosomes, Human, Pair 17, Fluorescence in situ hybridization |
| الوصف: | Background and Aim: In order to evaluate loss of the p53 gene more precisely, we performed dual-color fluorescence in situ hybridization (dual-color FISH) for chromosome 17 and p53 gene together with DNA polymorphism analysis of the p53 gene in hepatocellular carcinoma (HCC). Methods: Dual-color FISH using probes specific for the centromere of chromosome 17 and the p53 gene was performed for 41 HCC and DNA polymorphism analysis was also performed for them. Results: Of the 34 HCC tested by dual-color FISH, 20 had loss of at least one p53 gene (58.8%). In contrast, of the 32 HCC tested by DNA polymorphism analysis, 23 gave informative results, among which only eight had loss of heterozygosity (LOH) of the p53 gene (34.8%). Notably, among 14 cases positive for loss of the p53 gene by dual-color FISH, seven cases were negative for LOH of the p53 gene. Moreover, dual-color FISH revealed that the percentage of cells that lost at least one p53 gene increased as the HCC became less differentiated (P |
| تدمد: | 1440-1746 0815-9319 |
| DOI: | 10.1111/j.1440-1746.2004.03407.x |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b3cf64ef7e46178fdebe852d3924db2 https://doi.org/10.1111/j.1440-1746.2004.03407.x |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2b3cf64ef7e46178fdebe852d3924db2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14401746 08159319 |
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| DOI: | 10.1111/j.1440-1746.2004.03407.x |