SCASP: A Simple and Robust SDS-Aided Sample Preparation Method for Proteomic Research
| العنوان: | SCASP: A Simple and Robust SDS-Aided Sample Preparation Method for Proteomic Research |
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| المؤلفون: | Xiao Xu, Chuan-Qi Zhong, Guohong Gan, Jinling Wang, Xiu-Fang Zhang, Xi Chen |
| المصدر: | Molecular & Cellular Proteomics : MCP |
| بيانات النشر: | Elsevier BV, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Proteomics, Swath ms, BAEE, Nα-benzoyl-l-arginine ethyl ester hydrochloride, FDR, false discovery rate, Electrospray ionization, SCASP, SDS–CD-assisted sample preparation, SP3, single-pot and solid-phase–enhanced sample preparation, FFPE, formalin-fixed and paraffin-embedded, SDC, Biochemistry, Cell Line, Analytical Chemistry, TFA, trifluoroacetic acid, 03 medical and health sciences, SDB-RPS, poly(styrenedivinylbenzene)-reversed phase sulfonate, FASP, filter-aided sample preparation, Animals, Humans, iRT, independent retention time, Trypsin, Sample preparation, SWATH-MS, Sequential Window Acquisition of All Theoretical Mass Spectra, SDS, TCEP, Tris(2-carboxyethyl)phosphine hydrochloride, Molecular Biology, Sodium Deoxycholate, FA, formic acid, 030304 developmental biology, Cyclodextrins, CAA, 2-chloroacetamide, 0303 health sciences, Chromatography, sample preparation, Chemistry, 030302 biochemistry & molecular biology, Technological Innovation and Resources, Sodium Dodecyl Sulfate, ACN, acetonitrile, PASEF, parallel accumulation—serial fragmentation, Mice, Inbred C57BL, CD, cyclodextrin, iST, in-StageTip, DDA, data-dependent acquisition, SDC, sodium deoxycholate |
| الوصف: | SDS is widely used in sample preparation for proteomic research. However, SDS is incompatible with LC and electrospray ionization. SDS depletion is therefore required ahead of LC–MS analysis. Most of current SDS removal strategies are time consuming, laborious, and have low reproducibility. Here, we describe a method, SDS–cyclodextrin (CD)-assisted sample preparation, by which CD can bind to SDS and form CD–SDS complexes in solutions, allowing for direct tryptic digestion. We demonstrate that SDS–CD-assisted sample preparation is a simple, fast, and robust SDS-based sample preparation method for proteomics application. Graphical Abstract Highlights • SCASP can effectively process SDS-containing samples. • SCASP is robust and easy to implement. • Superior performance of SCASP compared with current SDS-based methods. • SCASP is suitable for preparation of challenging samples. In Brief Cyclodextrins can be used for removal of SDS in protein solution, facilitating direct tryptic digestion. The SDS–cyclodextrin-assisted sample preparation method is a simple, robust, and reproducible strategy for proteomics research and highly suitable for challenging samples. |
| تدمد: | 1535-9476 |
| DOI: | 10.1016/j.mcpro.2021.100051 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ab107db5b74bd878942e830c6b81ec1 https://doi.org/10.1016/j.mcpro.2021.100051 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2ab107db5b74bd878942e830c6b81ec1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15359476 |
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| DOI: | 10.1016/j.mcpro.2021.100051 |