Molecular analysis of critical sequences within the EBNA-2 type 1 gene from Epstein-Barr virus isolates from patients with infectious mononucleosis, tonsillar hyperplasia, and HIV infection
| العنوان: | Molecular analysis of critical sequences within the EBNA-2 type 1 gene from Epstein-Barr virus isolates from patients with infectious mononucleosis, tonsillar hyperplasia, and HIV infection |
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| المؤلفون: | D van Baarle, M R Klein, C McQuain, A S Al-Homsi, M J Kersten, Hans Knecht, Christoph Berger, R van Oers |
| المساهمون: | Translational Immunology Groningen (TRIGR), Other departments |
| المصدر: | International journal of molecular medicine, 1(6), 983-987. SPANDIDOS PUBL LTD International journal of molecular medicine, 1(6), 983-987. Spandidos Publications |
| سنة النشر: | 1998 |
| مصطلحات موضوعية: | Herpesvirus 4, Human, Mononucleosis, Herpesvirus 4, Human/genetics, DNA Mutational Analysis, Palatine Tonsil, HIV Infections, Palatine Tonsil/pathology, Biology, medicine.disease_cause, Virus, Viral Proteins, hemic and lymphatic diseases, Insertional, Genetics, medicine, Viral/chemistry, Tumor Cells, Cultured, Humans, Point Mutation, Infectious Mononucleosis, Child, Gene, Human/genetics, Cultured, Hyperplasia, Oncogene, Point mutation, Herpesvirus 4, General Medicine, DNA, medicine.disease, Epstein–Barr virus, Virology, Molecular medicine, HIV Infections/pathology, Tumor Cells, DNA, Viral/chemistry, Mutagenesis, Insertional, Epstein-Barr Virus Nuclear Antigens, Mutagenesis, Immunology, Viral Proteins/genetics, DNA, Viral, Infectious Mononucleosis/pathology |
| الوصف: | EBNA-2 is the first protein to be detected after infection of primary B lymphocytes by Epstein-Barr virus (EBV) and plays an essential role as transcriptional activator in EBV-induced lymphocyte transformation. We analysed by PCR and sequencing regions of the EBNA-2 type 1 gene from isolates from 13 children with infectious mononucleosis (IM), 6 children with tonsillar hyperplasia (TH), and 9 patients with HIV infection followed longitudinally. We found in all three groups of patients frequent non-silent point mutations at positions 48990, 48991, 49021, 49057, 49083, 49089, 49091, 49113, 49119, 49140, 49156, and a triplet insertion at position 49136. While 4 out of 13 samples from patients with IM showed a mosaic pattern suggesting co-existence of more than 1 substrain of EBNA-2 type 1, none of the samples from TH showed this pattern consistent with substrain selection during clinical latency. No sequence changes were noted over time in samples derived from patients with HIV infection. We conclude that in analogy to the coexistence of several subtypes of EBNA-1 in healthy EBV carriers, samples from IM can harbor more than one subtype of the EBNA-2 type 1 gene. |
| تدمد: | 1107-3756 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a66306ac52f69468ac4046240227a1e https://pubmed.ncbi.nlm.nih.gov/9852635 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2a66306ac52f69468ac4046240227a1e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 11073756 |
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