Differential Activation of Partially Redundant Δ9 Stearoyl-ACP Desaturase Genes Is Critical for Omega-9 Monounsaturated Fatty Acid Biosynthesis During Seed Development in Arabidopsis
| العنوان: | Differential Activation of Partially Redundant Δ9 Stearoyl-ACP Desaturase Genes Is Critical for Omega-9 Monounsaturated Fatty Acid Biosynthesis During Seed Development in Arabidopsis |
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| المؤلفون: | Delphine De Vos, Guillaume Barthole, Damien Vasselon, Sami Kazaz, Hasna Ettaki, Alexandra To, Loïc Lepiniec, Sébastien Baud, Frédéric Domergue, Katia Belcram |
| المساهمون: | Institut national de la recherche agronomique [Maroc] (INRA Maroc), Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire de biogenèse membranaire (LBM), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), ANR-10-GENM-0009,SOLAR,Développer et tester de nouveaux outils biotechnologiques pour accroître la teneur en huile de la graine : une clé pour la production de chaînes hydrocarbonées chez les oléagineux.(2010), ANR-17-EURE-0007,SPS-GSR,Ecole Universitaire de Recherche de Sciences des Plantes de Paris-Saclay(2017) |
| المصدر: | The Plant cell The Plant cell, American Society of Plant Biologists (ASPB), 2020, 32 (11), pp.3613-3637. ⟨10.1105/tpc.20.00554⟩ Plant Cell |
| بيانات النشر: | HAL CCSD, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Fatty Acid Desaturases, 0106 biological sciences, [SDV]Life Sciences [q-bio], Mutant, Arabidopsis, Plant Science, 01 natural sciences, In Brief, Mixed Function Oxygenases, Endosperm, Fatty Acids, Monounsaturated, 03 medical and health sciences, chemistry.chemical_compound, Gene Expression Regulation, Plant, Arabidopsis thaliana, Gene, Research Articles, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, biology, Arabidopsis Proteins, food and beverages, Fatty acid, Embryo, Cell Biology, Plants, Genetically Modified, biology.organism_classification, Lipids, Cell biology, Oleic acid, chemistry, Mutation, Seeds, lipids (amino acids, peptides, and proteins), Transcription Factors, 010606 plant biology & botany |
| الوصف: | The spatiotemporal pattern of deposition, final amount, and relative abundance of oleic acid (cis-ω-9 C18:1) and its derivatives in the different lipid fractions of the seed of Arabidopsis (Arabidopsis thaliana) indicates that omega-9 monoenes are synthesized at high rates in this organ. Accordingly, we observed that four Δ9 stearoyl-ACP desaturase (SAD)-coding genes (FATTY ACID BIOSYNTHESIS2 [FAB2], ACYL-ACYL CARRIER PROTEIN5 [AAD5], AAD1, and AAD6) are transcriptionally induced in seeds. We established that the three most highly expressed ones are directly activated by the WRINKLED1 transcription factor. We characterized a collection of 30 simple, double, triple, and quadruple mutants affected in SAD-coding genes and thereby revealed the functions of these desaturases throughout seed development. Production of oleic acid by FAB2 and AAD5 appears to be critical at the onset of embryo morphogenesis. Double homozygous plants from crossing fab2 and aad5 could never be obtained, and further investigations revealed that the double mutation results in the arrest of embryo development before the globular stage. During later stages of seed development, these two SADs, together with AAD1, participate in the elaboration of the embryonic cuticle, a barrier essential for embryo–endosperm separation during the phase of invasive embryo growth through the endosperm. This study also demonstrates that the four desaturases redundantly contribute to storage lipid production during the maturation phase. |
| اللغة: | English |
| تدمد: | 1040-4651 1532-298X |
| DOI: | 10.1105/tpc.20.00554⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28310117b2e0ecdb315c4f0a75294368 https://hal.archives-ouvertes.fr/hal-03004097 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....28310117b2e0ecdb315c4f0a75294368 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10404651 1532298X |
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| DOI: | 10.1105/tpc.20.00554⟩ |