Rapid cytotoxicity of antimicrobial peptide tempoprin-1CEa in breast cancer cells through membrane destruction and intracellular calcium mechanism
| العنوان: | Rapid cytotoxicity of antimicrobial peptide tempoprin-1CEa in breast cancer cells through membrane destruction and intracellular calcium mechanism |
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| المؤلفون: | Li-Jie Ma, Song Li, Che Wang, Yang Zhou, Li-Li Tian, He Wang, De-Jing Shang, Qing-Zhu Yang, Hui-Bing Li |
| المصدر: | PLoS ONE, Vol 8, Iss 4, p e60462 (2013) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Peptide Nucleic Acids, Time Factors, Cancer Treatment, Intracellular Space, lcsh:Medicine, Biochemistry, Calcium in biology, Synthetic Nucleic Acids, chemistry.chemical_compound, Cytosol, Nucleic Acids, Molecular Cell Biology, Breast Tumors, Cytotoxicity, lcsh:Science, Energy-Producing Organelles, Membrane potential, Membrane Potential, Mitochondrial, Multidisciplinary, Cell Death, Obstetrics and Gynecology, Depolarization, Phosphatidylserine, Cellular Structures, Cell biology, Oncology, Cytochemistry, Medicine, Research Article, Programmed cell death, Biophysics, Antineoplastic Agents, Breast Neoplasms, Phosphatidylserines, Biology, Bioenergetics, Permeability, Cell Line, Tumor, Breast Cancer, Humans, MTT assay, Organelles, Cell Membrane, lcsh:R, Cancers and Neoplasms, chemistry, Subcellular Organelles, Cancer cell, Calcium, lcsh:Q, Reactive Oxygen Species, Antimicrobial Cationic Peptides |
| الوصف: | Temporin-1CEa is an antimicrobial peptide isolated from the skin secretions of the Chinese brown frog (Rana chensinensis). We have previously reported the rapid and broad-spectrum anticancer activity of temporin-1CEa in vitro. However, the detailed mechanisms for temporin-1CEa-induced cancer cell death are still weakly understood. In the present study, the mechanisms of temporin-1CEa-induced rapid cytotoxicity on two human breast cancer cell lines, MDA-MB-231 and MCF-7, were investigated. The MTT assay and the LDH leakage assay indicated that one-hour of incubation with temporin-1CEa led to cytotoxicity in a dose-dependent manner. The morphological observation using electronic microscopes suggested that one-hour exposure of temporin-1CEa resulted in profound morphological changes in both MDA-MB-231 and MCF-7 cells. The membrane-disrupting property of temporin-1CEa was further characterized by induction of cell-surface exposure of phosphatidylserine, elevation of plasma membrane permeability and rapid depolarization of transmembrane potential. Moreover, temporin-1CEa evoked intracellular calcium ion and reactive oxygen species (ROS) elevations as well as collapse of mitochondrial membrane potential (Δφm). In summary, the present study indicates that temporin-1CEa triggers rapid cell death in breast cancer cells. This rapid cytotoxic activity might be mediated by both membrane destruction and intracellular calcium mechanism. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27e1ac2381aa6aaa2bd78198f3d879ae http://europepmc.org/articles/PMC3618425?pdf=render |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....27e1ac2381aa6aaa2bd78198f3d879ae |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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