Comparison of the characteristics of Mycobacterium tuberculosis isolates from sputum and lung lesions in chronic tuberculosis patients
| العنوان: | Comparison of the characteristics of Mycobacterium tuberculosis isolates from sputum and lung lesions in chronic tuberculosis patients |
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| المؤلفون: | H.-K. Kwak, J.-H. Kim, S.-Y. Eum, J.-S. Lee, Y.-I. Choi, M.-S. Hong, H. Lee, Yeon Sook Kim, E.-J. Cho, C.-T. Kim, J.-S. Cho, S.-K. Park, D. Jeon |
| المصدر: | European Journal of Clinical Microbiology & Infectious Diseases. 36:2063-2072 |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Microbiology (medical), medicine.medical_specialty, Tuberculosis, 030106 microbiology, Antitubercular Agents, Microbial Sensitivity Tests, Drug resistance, Gastroenterology, Mycobacterium tuberculosis, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Drug Resistance, Multiple, Bacterial, Internal medicine, Tuberculosis, Multidrug-Resistant, medicine, Humans, 030212 general & internal medicine, Typing, Lung, Tuberculosis, Pulmonary, Genotyping, biology, business.industry, Sputum, General Medicine, Middle Aged, respiratory system, biology.organism_classification, medicine.disease, respiratory tract diseases, Infectious Diseases, medicine.anatomical_structure, Immunology, Female, medicine.symptom, Restriction fragment length polymorphism, business, Polymorphism, Restriction Fragment Length |
| الوصف: | Mycobacterium tuberculosis (Mtb) in sputum originates from lung cavities in tuberculosis (TB) patients. But drug susceptibility testing (DST) of sputum Mtb can not be conducted the same as in the lung because mutagenesis of bacilli may be happening in the lung during treatment and result in the possibility of the presence of heterogeneous drug-resistant subpopulations in the different lung lesions. This could be one of the reasons for low cure rates for multi-drug resistant (MDR)-TB. We studied the resected lungs of nine surgery patients with chronic TB. The isolates isolated from the sputum and different lung lesions of each patient were tested for phenotypic DST and genotyped using restriction fragment length polymorphism (RFLP) typing method. Genetic analysis to resistance to first and second line drugs was also performed. Five of nine patients were MDR-TB and three XDR-TB. DST results for ten anti-TB drugs were in accordance among different lung lesions in eight patients. However, only three of these eight patients showed the concordance of DST with sputum. Even though the isolates were heteroresistant, genotyping them by RFLP showed the clonal population in each individual patient. Six of eight followed-up patients achieved successful cure. In conclusion, the heteroresistance between sputum and lung lesions and a clonal population without mixed infection might provide useful information in establishing treatment regimen and surgery decision for MDR- and XDR-TB. |
| تدمد: | 1435-4373 0934-9723 |
| DOI: | 10.1007/s10096-017-3025-5 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27bfb30a939eb7e7be6a6a3f5586125d https://doi.org/10.1007/s10096-017-3025-5 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....27bfb30a939eb7e7be6a6a3f5586125d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14354373 09349723 |
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| DOI: | 10.1007/s10096-017-3025-5 |