This study aimed to assess solar radiation transmission and animal thermal comfort indexes in two silvopastoral systems established with different tree arrangements in a tropical region. This study was conducted between 2014 and 2017 in two silvopastoral systems, one composed by an established Urochloa (syn. Brachiaria) decumbens pasture with Brazilian native trees planted in triple rows spaced 17 m apart, and another by an established Urochloa (syn. Brachiaria) brizantha (Hochst ex A. Rich.) Stapf ‘BRS Piata’ pasture with Eucalyptus urograndis (clone GG100) trees arranged in single rows spaced 15 m apart. In these systems and in a full-sun pasture, photosynthetically active radiation transmission, air temperature, relative humidity, black globe temperature, and wind speed were measured. These variables were used to calculate black globe temperature and humidity index (BGHI) and radiant thermal load (RTL). Higher animal thermal comfort was observed in the silvopastoral systems due to changes in the microclimate induced by the trees; notably, a decrease in solar radiation transmission. Fewer hours of potential animal thermal stress (BGHI>79) were observed in the silvopastoral systems than under the full-sun conditions, with differences up to 3 h per day. The silvopastoral systems presented lower radiant thermal load than the full-sun pasture with differences up to 22% achieved. The assessed silvopastoral systems may help livestock adapt to climate change, since they achieved the limit of BGHI considered to cause stress to animals following an increase of 2.2 °C in air temperature, compared with full-sun pastures.