Long-term in vivo depletion of functional CD4+ T lymphocytes from calves requires both thymectomy and anti-CD4 monoclonal antibody treatment
| العنوان: | Long-term in vivo depletion of functional CD4+ T lymphocytes from calves requires both thymectomy and anti-CD4 monoclonal antibody treatment |
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| المؤلفون: | Reginald Valdez, Donald P. Knowles, William C. Davis, Wendy C. Brown, Travis C. McGuire |
| المصدر: | Immunology. 102:426-433 |
| بيانات النشر: | Wiley, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | CD4-Positive T-Lymphocytes, Male, Anti-CD4 Monoclonal Antibody, Ovalbumin, medicine.medical_treatment, Immunology, Cell Culture Techniques, Spleen, Thymus Gland, Immunoglobulin G, Mice, In vivo, medicine, Animals, Immunology and Allergy, biology, Antibodies, Monoclonal, Original Articles, T lymphocyte, Thymectomy, medicine.anatomical_structure, CD4 Antigens, biology.protein, Cattle, Lymph Nodes, Antibody, Immunologic Memory, Cell Division |
| الوصف: | In vivo depletion of lymphocyte subsets is a direct approach used for dissection of the mechanisms of protective immunity. Long-term in vivo depletion of bovine T lymphocyte subpopulations with monoclonal antibody (mAb) treatment alone has been difficult to achieve. The objective of this study was to determine whether both thymectomy and anti-CD4 mAb treatment would optimize long-term in vivo depletion of functional bovine CD4+ T lymphocytes. Calves were thymectomized and treated with high doses of anti-CD4 mAb (approximately 5 mg/kg) over 4 days followed by subsequent lower doses (approximately 0·3 mg/kg) administered twice weekly for an additional 7 weeks. Depletion of CD4+ T lymphocytes from blood, spleen and peripheral lymph nodes was significantly improved in thymectomized calves compared to thymus-intact anti-CD4 mAb-treated calves. Significant differences in percentages of CD4+ T lymphocytes between thymectomized and thymus-intact calves were sustained for the duration of the 8-week study. Depletion of CD4+ T lymphocytes from thymectomized calves resulted in complete abrogation of lymphoproliferative responses to ovalbumin. In addition, thymectomized calves treated with anti-CD4 mAb had significantly reduced immunoglobulin G1 and no detectable immunoglobulin G2 ovalbumin-specific antibody responses compared to thymus-intact anti-CD4 mAb-treated calves. The results of this study demonstrate that both thymectomy and treatment with anti-CD4 mAb are required for long-term in vivo depletion of functional bovine CD4+ T lymphocytes. |
| تدمد: | 1365-2567 0019-2805 |
| DOI: | 10.1046/j.1365-2567.2001.01211.x |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26401bb79403d8bb20779463601427ef https://doi.org/10.1046/j.1365-2567.2001.01211.x |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....26401bb79403d8bb20779463601427ef |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652567 00192805 |
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| DOI: | 10.1046/j.1365-2567.2001.01211.x |