Melanin in Fonsecaea pedrosoi: a trap for oxidative radicals
| العنوان: | Melanin in Fonsecaea pedrosoi: a trap for oxidative radicals |
|---|---|
| المؤلفون: | Marcelo H. Herbst, Sonia Rozental, Luana P Borba, Anderson J. Franzen, Sergio Henrique Seabra, N.V. Vugman, Marcel M.L. Cunha, Wanderley de Souza |
| المصدر: | BMC Microbiology, Vol 10, Iss 1, p 80 (2010) BMC Microbiology |
| بيانات النشر: | BMC, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Microbiology (medical), Free Radicals, lcsh:QR1-502, Nitric Oxide Synthase Type II, Oxidative phosphorylation, medicine.disease_cause, Nitric Oxide, Microbiology, lcsh:Microbiology, Nitric oxide, Melanin, chemistry.chemical_compound, Mice, Ascomycota, Research article, medicine, Animals, Microscopy, Phase-Contrast, Microwaves, Nitrites, Cell Proliferation, Melanins, biology, integumentary system, Macrophages, Electron Spin Resonance Spectroscopy, Hydrogen Peroxide, Pathogenic fungus, biology.organism_classification, Immunohistochemistry, Respiratory burst, Nitric oxide synthase, Fonsecaea pedrosoi, Thiazoles, Biochemistry, chemistry, Host-Pathogen Interactions, biology.protein, sense organs, Oxidative stress |
| الوصف: | Background The pathogenic fungus Fonsecaea pedrosoi constitutively produces the pigment melanin, an important virulence factor in fungi. Melanin is incorporated in the cell wall structure and provides chemical and physical protection for the fungus. We evaluated the production of nitric oxide (NO) in macrophages, the oxidative burst and the inducible nitric oxide synthase (i-NOS) activity in interactions between activated murine macrophages and F. pedrosoi. Experiments were carried out with or without tricyclazole (TC) treatment, a selective inhibitor of the dihydroxynaphthalene (DHN)-melanin biosynthesis pathway in F. pedrosoi. The paramagnetisms of melanin and the TC-melanin were analysed by electron spin resonance. The fungal growth responses to H2O2 and to S-nitroso-N-acetylpenicillamine (SNAP), a nitric oxide donor, were also evaluated. Results Melanised F. pedrosoi cells were more resistant to both H2O2 and NO. Nitrite was not detected in the supernatant of macrophages incubated with melanised fungal cells. However, i-NOS expression was unaffected by the presence of either untreated control F. pedrosoi or TC-treated F. pedrosoi. In addition, the inhibition of the DHN-melanin pathway by TC improved the oxidative burst capability of the macrophages. Conclusion The NO-trapping ability of F. pedrosoi melanin is an important mechanism to escape the oxidative burst of macrophages. |
| اللغة: | English |
| تدمد: | 1471-2180 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::260cd1febf5d96a0aaaa4ce908529585 http://www.biomedcentral.com/1471-2180/10/80 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....260cd1febf5d96a0aaaa4ce908529585 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712180 |
|---|