A regulatory gene induces trichome formation and embryo lethality in tomato
| العنوان: | A regulatory gene induces trichome formation and embryo lethality in tomato |
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| المؤلفون: | Zhibiao Ye, Hanxia Li, Pengjuan Gong, Zhidan Luo, Lu Yong'en, Yuyang Zhang, Taotao Wang, Chanjuan Zhang, Changxian Yang, Junhong Zhang, Jinhua Li |
| المصدر: | Proceedings of the National Academy of Sciences. 108:11836-11841 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Genetic Vectors, Mutation, Missense, Biology, Genes, Plant, Plant Epidermis, Solanum lycopersicum, Gene Expression Regulation, Plant, RNA interference, Cyclins, Two-Hybrid System Techniques, Cloning, Molecular, Gene, In Situ Hybridization, Regulator gene, Homeodomain Proteins, Regulation of gene expression, Multidisciplinary, Epidermis (botany), Microarray analysis techniques, Gene Expression Profiling, Cell Differentiation, Biological Sciences, Microarray Analysis, Plants, Genetically Modified, Molecular biology, Trichome, Gene expression profiling, Blotting, Southern, Microscopy, Electron, Scanning, RNA Interference |
| الوصف: | Trichomes are universal biological structures originating from the aerial epidermis, which serve as an excellent model to study plant differentiation at the cell level. Although the pathway regulating trichome formation in the Rosids has been well characterized, only very recently a few genes were identified for trichome initiation in the Asterids. In this study, we cloned Woolly ( Wo ), essential for trichome formation in tomato. Transgenic experiments revealed that the woolly phenotype is caused by the mutation in Wo which encodes a homeodomain protein containing a bZIP motif and a START domain. We identified three alleles of Wo and found that each allele contains a missense mutation, which respectively results in an amino acid substitution at the C terminus. Microarray and expression analysis showed that the expression of a B-type cyclin gene, SlCycB2 , is possibly regulated by Wo , which also participates in trichome formation. Suppression of Wo or SlCycB2 expression by RNAi decreased the number of type I trichomes, and direct protein–protein interaction was detected between them, implying that both proteins may work together in the regulation of this type of trichome formation. Cytological observation and Wo transcript analysis in the developing seeds showed that embryo development was also correlated with Wo . |
| تدمد: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1100532108 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25351bdab6bd9258e2f62b4c31e44482 https://doi.org/10.1073/pnas.1100532108 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....25351bdab6bd9258e2f62b4c31e44482 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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| DOI: | 10.1073/pnas.1100532108 |