A sensitive real-time PCR for detection and subgrouping of human respiratory syncytial virus
| العنوان: | A sensitive real-time PCR for detection and subgrouping of human respiratory syncytial virus |
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| المؤلفون: | H. Rogier van Doorn, Menno D. de Jong, Lien Anh Ha Do, Jeremy Farrar, Tinh Hien Tran, Minh Nguyen, Cong Khanh Vo, Vinh Chau Nguyen Van, Juliet E. Bryant, My Ngoc Nghiem |
| المساهمون: | Medical Microbiology and Infection Prevention, Amsterdam institute for Infection and Immunity |
| المصدر: | Journal of Virological Methods Journal of virological methods, 179(1), 250-255. Elsevier |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Paramyxoviridae, viruses, Respiratory Syncytial Virus Infections, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Severity of Illness Index, Article, Virus, 03 medical and health sciences, Pneumovirinae, Virology, Humans, Multiplex, Locked nucleic acid, Child, Mononegavirales, 030304 developmental biology, 0303 health sciences, biology, 030306 microbiology, Infant, Newborn, Infant, RSV, virus diseases, Viral Load, respiratory system, biology.organism_classification, 3. Good health, LNA, Real-time polymerase chain reaction, Molecular Diagnostic Techniques, Vietnam, Child, Preschool, Respiratory Syncytial Virus, Human, Immunology, Oligonucleotide Probes, Viral load, Real-time PCR |
| الوصف: | Highlights ► A novel real-time RT-PCR using specific locked nucleic acid probes is described. ► The assay is quantitative and distinguishes RSV subgroup A & B. ► Compared with a commercial multiplex PCR using 264 respiratory samplesin Vietnam. ► Sensitivity was significantly higher with detection a rate of 32 vs. 24%. Improved diagnostic tools for rapid detection, quantitation, and subgrouping of human respiratory syncytial virus (RSV) are needed to aid the development and evaluation of novel intervention strategies. A quantitative real-time RT-PCR using specific locked nucleic acid (LNA) probes was developed to identify RSV and to distinguish RSV subgroups A and B (RSV LNA assay). RSV subgroup diversity and the relationship between viral load and disease severity in confirmed RSV infections were also explored. 264 archived respiratory specimens from pediatric patients were tested in parallel using the commercial multiplex Seeplex™ RV detection kit (Seegene) and the novel RSV LNA assay. The LNA assay demonstrated a significantly higher sensitivity than Seeplex, improving overall detection rates from 24% (64/264) to 32% (84/264). Detection limits of 9.0 × 101 and 6.0 × 102 copies/mL were observed for RSV A and B, respectively. RSV A was detected in 53/84 (63%) cases, and 31/84 (37%) were positive for RSV B. This novel method offers a rapid, quantitative, highly specific and sensitive approach to laboratory diagnosis of RSV. |
| اللغة: | English |
| تدمد: | 0166-0934 |
| DOI: | 10.1016/j.jviromet.2011.11.012 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::250c35f305426982bdbc22ebc26df3a4 https://doi.org/10.1016/j.jviromet.2011.11.012 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....250c35f305426982bdbc22ebc26df3a4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 01660934 |
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| DOI: | 10.1016/j.jviromet.2011.11.012 |