Detection of low-frequency DNA variants by targeted sequencing of the Watson and Crick strands
| العنوان: | Detection of low-frequency DNA variants by targeted sequencing of the Watson and Crick strands |
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| المؤلفون: | Brian J. Mog, Kenneth W. Kinzler, Jeanne Tie, Maria Popoli, Lisa Dobbyn, Christopher Douville, Peter Gibbs, Bert Vogelstein, Nickolas Papadopoulos, Cristian Tomasetti, Natalie Silliman, Joy Schaefer, Jonathan C. Dudley, Janine Ptak, Joshua D. Cohen |
| المصدر: | Nat Biotechnol |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0303 health sciences, Mutation rate, Dna template, Biomedical Engineering, Word error rate, Bioengineering, Genomics, Molecular Structure of Nucleic Acids: A Structure for Deoxyribose Nucleic Acid, Computational biology, Biology, Dna variants, Applied Microbiology and Biotechnology, Genome, Article, DNA sequencing, 03 medical and health sciences, 0302 clinical medicine, Molecular Medicine, 030217 neurology & neurosurgery, 030304 developmental biology, Biotechnology |
| الوصف: | Identification and quantification of low-frequency mutations remain challenging despite improvements in the baseline error rate of next-generation sequencing technologies. Here, we describe a method, termed SaferSeqS, that addresses these challenges by (1) efficiently introducing identical molecular barcodes in the Watson and Crick strands of template molecules and (2) enriching target sequences with strand-specific PCR. The method achieves high sensitivity and specificity and detects variants at frequencies below 1 in 100,000 DNA template molecules with a background mutation rate of 100-fold. |
| تدمد: | 1546-1696 1087-0156 |
| DOI: | 10.1038/s41587-021-00900-z |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24fb58fbee1abcff3474200148e4d6dd https://doi.org/10.1038/s41587-021-00900-z |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....24fb58fbee1abcff3474200148e4d6dd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15461696 10870156 |
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| DOI: | 10.1038/s41587-021-00900-z |