High‐fat diet affects autophagy and mitochondrial compartment in rat Harderian gland
| العنوان: | High‐fat diet affects autophagy and mitochondrial compartment in rat Harderian gland |
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| المؤلفون: | Gabriella Chieffi Baccari, Sara Falvo, Maria M. Di Fiore, Federica Cioffi, Antonia Giacco, Alessandra Santillo |
| المساهمون: | Chieffi, Gabriella, Falvo, Sara, DI FIORE, Maria Maddalena, Cioffi, Federica, Giacco, Antonia, Santillo, Alessandra |
| المصدر: | Journal of Experimental Zoology Part A: Ecological and Integrative Physiology. 337:1025-1038 |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | autophagy, Harderian gland, Physiology, High-Fat Diet, Diet, High-Fat, DNA, Mitochondrial, Lipids, Rats, mitochondria, Genetics, Animals, Animal Science and Zoology, Molecular Biology, Ecology, Evolution, Behavior and Systematics |
| الوصف: | The Harderian gland (HG) of Rattus norvegicus is an orbital gland secreting lipids that accumulate in excess under condition of increased lipid metabolism. To study the response elicitated by lipid overload in rat HG, we housed the animals in thermoneutral conditions (28-30°C) in association to high fat diet (HFD). In HFD rats alterated blood lipid levels result in lipid accumulation in HG as demonstrated by the increased gland weight and histochemical/ultrastructural analyses. The HFD-caused oxidative stress forces the gland to trigger antioxidant defense mechanisms and autophagic process, such as lipophagy and mitophagy. Induction of mitochondrial DNA (mtDNA) damage and repair was stronger in HFD-rat HGs. An increase in marker expression levels of mitochondrial biogenesis, fission, and fusion occurred to counteract mtDNA copy number reduction and mitophagy. Therefore, the results demonstrate that rat HG activates autophagy as survival strategy under conditions of increased lipid metabolism and suggest a key role for mitophagy and membrane dynamics in the mitochondrial adaptive response to HFD. |
| تدمد: | 2471-5646 2471-5638 |
| DOI: | 10.1002/jez.2646 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::247e3c4bf5a5668fe69f3dad7bab4d2c https://doi.org/10.1002/jez.2646 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....247e3c4bf5a5668fe69f3dad7bab4d2c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 24715646 24715638 |
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| DOI: | 10.1002/jez.2646 |