Function of the plant DNA polymerase epsilon in replicative stress sensing, a genetic analysis
| العنوان: | Function of the plant DNA polymerase epsilon in replicative stress sensing, a genetic analysis |
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| المؤلفون: | Etienne Delannoy, Mickael Bourge, Iván del Olmo, Jose A. Jarillo, José Antonio Pedroza-Garcia, Séverine Domenichini, Moussa Benhamed, Manuel Piñeiro, Christelle Mazubert, Rémi Bounon, Catherine Bergounioux, Cécile Raynaud, Zakia Tariq |
| المساهمون: | Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11), Université Paris-Saclay, Université Sorbonne Paris Cité (USPC), Universidad Politécnica de Madrid (UPM), Institut de Biologie Intégrative de la Cellule (I2BC), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Université Paris Sud (Paris 11), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Agence Nationale de la Recherche (ANR) [ANR-2010-JCJC1207-01], Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), UMR 1403 Institut des Sciences des Plantes de Paris Saclay, Institut National de la Recherche Agronomique ( INRA ) -Université Paris Diderot - Paris 7 ( UPD7 ), Université Sorbonne Paris Cité ( USPC ), Universidad Politécnica de Madrid ( UPM ), Institut de Biologie Intégrative de la Cellule ( I2BC ), Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Sud - Paris 11 ( UP11 ), Commissariat à l'Energie Atomique et aux Energies Alternatives |
| المصدر: | Plant Physiology Plant Physiology, American Society of Plant Biologists, 2017, 173 (3), pp.1735-1749. ⟨10.1104/pp.17.00031⟩ Plant Physiology, 2017, 173 (3), pp.1735-1749. ⟨10.1104/pp.17.00031⟩ Repositorio de Resultados de Investigación del INIA Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA Plant Physiology, American Society of Plant Biologists, 2017, 173 (3), pp.1735-1749. 〈10.1104/pp.17.00031〉 INIA: Repositorio de Resultados de Investigación del INIA |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | DNA Replication, 0301 basic medicine, DNA, Plant, Physiology, DNA repair, DNA damage, DNA polymerase, [SDV]Life Sciences [q-bio], Arabidopsis, DNA polymerase epsilon, Plant Science, DNA polymerase delta, 03 medical and health sciences, chemistry.chemical_compound, Gene Expression Regulation, Plant, Genetics, Hydroxyurea, Polymerase, Nucleic Acid Synthesis Inhibitors, 2. Zero hunger, [ SDV ] Life Sciences [q-bio], Models, Genetic, biology, Arabidopsis Proteins, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, DNA replication, Cell Cycle Checkpoints, DNA Polymerase II, Articles, Plants, Genetically Modified, Gene Ontology, 030104 developmental biology, Microscopy, Fluorescence, chemistry, Mutation, biology.protein, RNA Interference, DNA |
| الوصف: | Faithful transmission of the genetic information is essential in all living organisms. DNA replication is therefore a critical step of cell proliferation, because of the potential occurrence of replication errors or DNA damage when progression of a replication fork is hampered causing replicative stress. Like other types of DNA damage, replicative stress activates the DNA damage response, a signaling cascade allowing cell cycle arrest and repair of lesions. The replicative DNA polymerase « (Pol «) was shown to activate the S-phase checkpoint in yeast in response to replicative stress, but whether this mechanism functions in multicellular eukaryotes remains unclear. Here, we explored the genetic interaction between Pol « and the main elements of the DNA damage response in Arabidopsis (Arabidopsis thaliana). We found that mutations affecting the polymerase domain of Pol « trigger ATRdependent signaling leading to SOG1 activation, WEE1-dependent cell cycle inhibition, and tolerance to replicative stress induced by hydroxyurea, but result in enhanced sensitivity to a wide range of DNA damaging agents. Using knock-down lines, we also provide evidence for the direct role of Pol « in replicative stress sensing. Together, our results demonstrate that the role of Pol « in replicative stress sensing is conserved in plants, and provide, to our knowledge, the first genetic dissection of the downstream signaling events in a multicellular eukaryote. © 2017 American Society of Plant Biologists. All rights reserved. |
| اللغة: | English |
| تدمد: | 0032-0889 1532-2548 |
| DOI: | 10.1104/pp.17.00031⟩ |
| DOI: | 10.1104/pp.17.00031〉 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23d1c011d37e73e0b4d4edfb4ea98ad5 https://hdl.handle.net/20.500.12792/3362 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....23d1c011d37e73e0b4d4edfb4ea98ad5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00320889 15322548 |
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| DOI: | 10.1104/pp.17.00031⟩ |