Monte Carlo characterization of parallelized fluorescence confocal systems imaging in turbid media

التفاصيل البيبلوغرافية
العنوان: Monte Carlo characterization of parallelized fluorescence confocal systems imaging in turbid media
المؤلفون: Arthur F. Gmitro, Andrew R. Rouse, Anthony A. Tanbakuchi
المصدر: Journal of biomedical optics. 14(4)
سنة النشر: 2009
مصطلحات موضوعية: Materials science, Light, Aperture, Confocal, Monte Carlo method, Biomedical Engineering, Physics::Optics, Models, Biological, Article, law.invention, Biomaterials, Optics, Confocal microscopy, law, Nephelometry and Turbidimetry, Microscopy, Image Interpretation, Computer-Assisted, Animals, Humans, Scattering, Radiation, Computer Simulation, Nipkow disk, Microscopy, Confocal, Models, Statistical, business.industry, Scattering, Astrophysics::Instrumentation and Methods for Astrophysics, Atomic and Molecular Physics, and Optics, Electronic, Optical and Magnetic Materials, Microscopy, Fluorescence, Subtraction Technique, Pinhole (optics), business, Monte Carlo Method
الوصف: We characterize and compare the axial and lateral performance of fluorescence confocal systems imaging in turbid media. The aperture configurations studied are a single pinhole, a slit, a Nipkow disk, and a linear array of pinholes. Systems with parallelized apertures are used clinically because they enable high-speed and real-time imaging. Understanding how they perform in highly scattering tissue is important. A Monte Carlo model was developed to characterize parallelized system performance in a scattering media representative of human tissues. The results indicate that a slit aperture has degraded performance, both laterally and axially. In contrast, the analysis reveals that multipinhole apertures such as a Nipkow disk or a linear pinhole array can achieve performance nearly equivalent to a single pinhole aperture. The optimal aperture spacing for the multipinhole apertures was determined for a specific tissue model. In addition to comparing aperture configurations, the effects of tissue nonradiative absorption, scattering anisotropy, and fluorophore concentration on lateral and axial performance of confocal systems were studied.
تدمد: 1560-2281
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2382e7742e87dbf4282f120d0d3b0d95
https://pubmed.ncbi.nlm.nih.gov/19725735
Rights: OPEN
رقم الانضمام: edsair.doi.dedup.....2382e7742e87dbf4282f120d0d3b0d95
قاعدة البيانات: OpenAIRE