Aim The purpose of this study was to evaluate and compare the flexural strength of commercially available acrylic (trevalone) and modified polymethylmethacrylate (PMMA). Materials and methods Four groups were tested; Group 1— control group regular MMA, group 2—2% methacrylic acid, 88% MMA, group 3—16% methacrylic acid, 84% MMA group 4— 20% methacrylic acid, 80% MMA 15 resin specimens of each group were polymerized. After processing, the specimens were subjected for flexural strength testing using three point bending test in a Universal Testing Machine. All data was statistically analyzed with one-way ANOVA, differences within the groups were analyzed by Scheffe's analysis. Results As the ratio of incorporated methacrylic acid to PMMA increased, the flexural strength decreased. Analysis of data revealed a significant decrease in flexural strength of specimens (p < 0.000) after incorporation of 12%, 16%, 20% methacrylic acid to heat polymerized acrylic resin, when compared with the control group. Lowest flexural strength was observed with specimens containing 20% methacrylic acid and highest flexural strength was observed with specimens containing conventional monomer without methacrylic acid. Conclusion It was observed that as the concentration of methacrylic acid in heat polymerized acrylic resin increases, the flexural strength decreases. Lowest flexural strength was observed with specimens containing 20% methacrylic acid and highest flexural strength was observed with specimens containing conventional monomer without methacrylic acid. Clinical significance The major advantages of addition of methacrylic acid to polymethylmethacrylate could be for the elderly people with restricted manual dexterity or cognitive disturbances, especially for patients who do not follow an adequate denture cleansing protocol and diabetic patients who are more susceptible for denture stomatitis. How to cite this article Jain T, Yadav NS, Pandita A, Feroz SMA, Kartika UK, Singh PP. A Comparative Evaluation of Flexural Strength of Commercially Available Acrylic and Modified Polymethylmethacrylate: An in vitro Study. J Contemp Dent Pract 2013;14(1):80-83.