SARS-CoV-2 infection and replication in human gastric organoids
| العنوان: | SARS-CoV-2 infection and replication in human gastric organoids |
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| المؤلفون: | Hans Clevers, Camilla Luni, Giovanni Giuseppe Giobbe, Anna Manfredi, Lucio Di Filippo, Matteo Pagliari, Vivian S. W. Li, Brendan C. Jones, Paolo De Coppi, Hannah T. Stuart, Francesco Bonfante, Valentina Panzarin, Nikhil Thapar, Alessio Bortolami, Onelia Gagliano, Silvia Perin, Alessandro Filippo Pellegata, Georg A. Busslinger, Davide Cacchiarelli, Elisa Zambaiti, Cecilia Laterza, Simon Eaton, Eva Mazzetto, Chiara Colantuono, Nicola Elvassore |
| المساهمون: | Giobbe, Giovanni Giuseppe, Bonfante, Francesco, Jones, Brendan C., Gagliano, Onelia, Luni, Camilla, Zambaiti, Elisa, Perin, Silvia, Laterza, Cecilia, Busslinger, Georg, Stuart, Hannah, Pagliari, Matteo, Bortolami, Alessio, Mazzetto, Eva, Manfredi, Anna, Colantuono, Chiara, Di Filippo, Lucio, Pellegata, Alessandro Filippo, Panzarin, Valentina, Thapar, Nikhil, Li, Vivian Sze Wing, Eaton, Simon, Cacchiarelli, Davide, Clevers, Han, Elvassore, Nicola, De Coppi, Paolo, Giobbe, G. G., Bonfante, F., Jones, B. C., Gagliano, O., Luni, C., Zambaiti, E., Perin, S., Laterza, C., Busslinger, G., Stuart, H., Pagliari, M., Bortolami, A., Mazzetto, E., Manfredi, A., Colantuono, C., Di Filippo, L., Pellegata, A. F., Panzarin, V., Thapar, N., Li, V. S. W., Eaton, S., Cacchiarelli, D., Clevers, H., Elvassore, N., De Coppi, P., Hubrecht Institute for Developmental Biology and Stem Cell Research |
| المصدر: | Nature Communications Nature Communications, 12(1). Nature Publishing Group Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021) |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Organoid, Virus Replication/physiology, General Physics and Astronomy, Aborted Fetu, CHILDREN, organoid, stomach, gastric epithelium, COVID, transcriptomic, Virus Replication, Transcriptome, 0302 clinical medicine, Interferon, Chlorocebus aethiops, Gastrointestinal models, Intestinal Mucosa, CYTOSCAPE, Child, Adult stem cells, 0303 health sciences, Multidisciplinary, Stomach, SARS-CoV-2/isolation & purification, digestive, oral, and skin physiology, food and beverages, EXPANSION, Stomach/pathology, Middle Aged, 3. Good health, Multidisciplinary Sciences, Organoids, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Child, Preschool, Aborted Fetus, Science & Technology - Other Topics, STEM-CELLS, medicine.drug, Human, Science, Biology, Chlorocebus aethiop, General Biochemistry, Genetics and Molecular Biology, Virus, Article, Cell Line, 03 medical and health sciences, COVID-19/pathology, medicine, Animals, Humans, Viral shedding, Aged, COVID-19, Infant, SARS-CoV-2, Preschool, 030304 developmental biology, Fetus, Science & Technology, Intestinal Mucosa/pathology, Animal, General Chemistry, MODEL, Organoids/pathology, Viral replication, Viral infection, Immunology, RNA |
| الوصف: | COVID-19 typically manifests as a respiratory illness, but several clinical reports have described gastrointestinal symptoms. This is particularly true in children in whom gastrointestinal symptoms are frequent and viral shedding outlasts viral clearance from the respiratory system. These observations raise the question of whether the virus can replicate within the stomach. Here we generate gastric organoids from fetal, pediatric, and adult biopsies as in vitro models of SARS-CoV-2 infection. To facilitate infection, we induce reverse polarity in the gastric organoids. We find that the pediatric and late fetal gastric organoids are susceptible to infection with SARS-CoV-2, while viral replication is significantly lower in undifferentiated organoids of early fetal and adult origin. We demonstrate that adult gastric organoids are more susceptible to infection following differentiation. We perform transcriptomic analysis to reveal a moderate innate antiviral response and a lack of differentially expressed genes belonging to the interferon family. Collectively, we show that the virus can efficiently infect the gastric epithelium, suggesting that the stomach might have an active role in fecal-oral SARS-CoV-2 transmission. Several clinical reports have described gastrointestinal symptoms for COVID-19, though whether the virus can replicate within the stomach remains unclear. Here the authors generate gastric organoids from human biopsies and show that the virus can efficiently infect gastric epithelium, suggesting that the stomach might have an active role in fecal-oral transmission. |
| وصف الملف: | ELETTRONICO; Electronic |
| اللغة: | English |
| تدمد: | 2041-1723 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::214a7a33c9ff862ebb1470fec7376cd4 http://hdl.handle.net/11585/838769 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....214a7a33c9ff862ebb1470fec7376cd4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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