Response Surface Optimized Ultrasonic-Assisted Extraction of Flavonoids from Sparganii Rhizoma and Evaluation of Their in Vitro Antioxidant Activities
| العنوان: | Response Surface Optimized Ultrasonic-Assisted Extraction of Flavonoids from Sparganii Rhizoma and Evaluation of Their in Vitro Antioxidant Activities |
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| المؤلفون: | Guangyun Chen, Qinan Wu, Wei Yue, Yanfang Wu, Xinsheng Wang, Qiaoli Liang |
| المصدر: | Molecules Molecules; Volume 17; Issue 6; Pages: 6769-6783 |
| بيانات النشر: | MDPI, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Antioxidant, antioxidant, DPPH, medicine.medical_treatment, ultrasound-assisted extraction, response surface methodology, sparganii rhizoma, flavonoids, Pharmaceutical Science, Typhaceae, Article, Antioxidants, Analytical Chemistry, chemistry.chemical_compound, Rutin, Superoxides, Drug Discovery, medicine, Formononetin, Ultrasonics, Physical and Theoretical Chemistry, Flavonoids, Chromatography, ABTS, Hydroxyl Radical, Organic Chemistry, Extraction (chemistry), Reproducibility of Results, Free Radical Scavengers, chemistry, Models, Chemical, Chemistry (miscellaneous), Molecular Medicine, Hydroxyl radical, Kaempferol, Rhizome, Drugs, Chinese Herbal |
| الوصف: | An efficient ultrasound-assisted extraction technique was employed to extract total flavonoids from Sparganii rhizoma. The optimum extraction conditions for the highest yield of total flavonoids were ethanol concentration 53.62%, ultrasonication time 29.41 min and ultrasound power 300 W, which were determined using response surface methodology. The extraction yields of the optimal ultrasound-assisted extraction were higher than using conventional extraction. The crude extract was then purified on a polyamide resin, whereby the flavonoids content in the purified extract increased to 94.62%. The antioxidant activities of the purified flavonoids including DPPH radical scavenging activity, ABTS+ radical scavenging activity, reducing power, hydroxyl radical scavenging activity and superoxide anion scavenging activity, were evaluated in vitro, which suggested that the flavonoids showed significant antioxidant activities. Rutin, kaempferol and formononetin were identified in the extract by comparing relative retention times and UV-Vis spectra with those of reference standards. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1420-3049 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20be62f020a03f2f2b1282757654abc3 http://europepmc.org/articles/PMC6268284 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....20be62f020a03f2f2b1282757654abc3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14203049 |
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