Inhibition of MYC attenuates tumor cell self‐renewal and promotes senescence in SMARCB1‐deficient Group 2 atypical teratoid rhabdoid tumors to suppress tumor growth in vivo
| العنوان: | Inhibition of MYC attenuates tumor cell self‐renewal and promotes senescence in SMARCB1‐deficient Group 2 atypical teratoid rhabdoid tumors to suppress tumor growth in vivo |
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| المؤلفون: | Laura Soucek, Andrew M. Donson, Etienne Danis, Andrea Griesinger, Rajeev Vibhakar, Diane K. Birks, Sujatha Venkataraman, Mariarita Santi, Nicholas K. Foreman, Angela Pierce, Irina Alimova |
| المصدر: | International Journal of Cancer. 144:1983-1995 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Cancer Research, Transgene, Mice, Nude, Biology, medicine.disease_cause, Chromatin remodeling, Proto-Oncogene Proteins c-myc, BET inhibitor, Mice, 03 medical and health sciences, 0302 clinical medicine, Cell Line, Tumor, medicine, Animals, Humans, Cell Self Renewal, SMARCB1, Cellular Senescence, Rhabdoid Tumor, Phenocopy, Teratoma, Azepines, SMARCB1 Protein, Triazoles, Xenograft Model Antitumor Assays, Embryonic stem cell, Chromatin, Gene Expression Regulation, Neoplastic, Cell Transformation, Neoplastic, Oncology, Cell culture, 030220 oncology & carcinogenesis, Bone Morphogenetic Proteins, Cancer research, Female, Carcinogenesis |
| الوصف: | Loss of SMARCB1 is the hallmark genetic event that characterizes rhabdoid tumors in children. Rhabdoid tumors of the brain (ATRT) occur in young children and are particularly challenging with poor long-term survival. SMARCB1 is a member of the SWI/SNF chromatin remodeling complex that is responsible for determining cellular pluripotency and lineage commitment. The mechanisms by which SMARCB1 deletion results in tumorigenesis remain unclear. Recent studies demonstrate that ATRT consists of 3 genomic subgroups with a subset of poor outcome tumors expressing high BMP and MYC pathway activation. Here we show that MYC occupies distinct promoter loci in ATRT compared to embryonic stem (ES) cells. Furthermore, using human ATRT cell lines, patient-derived cell culture, ex vivo patient-derived tumor, and orthotopic xenograft models, we show that MYC inhibition is a molecular vulnerability in SMARCB1-deleted tumors and that such inhibition effectively suppresses BMP and pluripotency-associated genomic programs, attenuates tumor cell self-renewal, promotes senescence, and inhibits ATRT tumor growth in vivo. Transgenic expression of Omomyc (a bona-fide MYC dominant negative) or chemical inhibition of MYC transcriptomic programs with the BET inhibitor JQ1 phenocopy genetic depletion of MYC, effectively restricting ATRT tumor growth and opening a promising therapeutic avenue for rhabdoid tumors in children. |
| تدمد: | 1097-0215 0020-7136 |
| DOI: | 10.1002/ijc.31873 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20ab5c72af6fa1dec7c4153fbbcf3300 https://doi.org/10.1002/ijc.31873 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....20ab5c72af6fa1dec7c4153fbbcf3300 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970215 00207136 |
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| DOI: | 10.1002/ijc.31873 |