New red-fluorescent calcium indicators for opto-genetics, photoactivation and multi-color imaging
| العنوان: | New red-fluorescent calcium indicators for opto-genetics, photoactivation and multi-color imaging |
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| المؤلفون: | Anne Feltz, Jean-Maurice Mallet, Mayeul Collot, Martin Oheim, Marcel van 't Hoff, Alsu I. Zamaleeva |
| المساهمون: | Institut de biologie de l'ENS Paris (IBENS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC), Institut de biologie de l'ENS Paris (UMR 8197/1024) (IBENS), Département de Biologie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL) |
| المصدر: | BBA-Biochimica et Biophysica Acta BBA-Biochimica et Biophysica Acta, Elsevier, 2014, 1843 (10), pp. 2284-2306. ⟨10.1016/j.bbamcr.2014.03.010⟩ BBA-Biochimica et Biophysica Acta, 2014, 1843 (10), pp. 2284-2306. ⟨10.1016/j.bbamcr.2014.03.010⟩ |
| بيانات النشر: | HAL CCSD, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Yellow fluorescent protein, Boron Compounds, Photochemistry, Green Fluorescent Proteins, Analytical chemistry, chemistry.chemical_element, Calcium, Optogenetics, 010402 general chemistry, 01 natural sciences, Fluorescence, Green fluorescent protein, Photostimulation, 03 medical and health sciences, Bacterial Proteins, Spectral cross-talk, Animals, Humans, Molecular Biology, 030304 developmental biology, Benzofurans, Fluorescent Dyes, 0303 health sciences, biology, Rhodamines, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Imidazoles, Cell Biology, 0104 chemical sciences, Molecular Imaging, Topical review, Luminescent Proteins, Spectrometry, Fluorescence, chemistry, biology.protein, Biophysics, Thermodynamics, Color imaging, Nanobiosensor, Genetically encoded calcium indicator (GECI) |
| الوصف: | Most chemical and, with only a few exceptions, all genetically encoded fluorimetric calcium (Ca(2+)) indicators (GECIs) emit green fluorescence. Many of these probes are compatible with red-emitting cell- or organelle markers. But the bulk of available fluorescent-protein constructs and transgenic animals incorporate green or yellow fluorescent protein (GFP and YFP respectively). This is, in part, not only heritage from the tendency to aggregate of early-generation red-emitting FPs, and due to their complicated photochemistry, but also resulting from the compatibility of green-fluorescent probes with standard instrumentation readily available in most laboratories and core imaging facilities. Photochemical constraints like limited water solubility and low quantum yield have contributed to the relative paucity of red-emitting Ca(2+) probes compared to their green counterparts, too. The increasing use of GFP and GFP-based functional reporters, together with recent developments in optogenetics, photostimulation and super-resolution microscopies, has intensified the quest for red-emitting Ca(2+) probes. In response to this demand more red-emitting chemical and FP-based Ca(2+)-sensitive indicators have been developed since 2009 than in the thirty years before. In this topical review, we survey the physicochemical properties of these red-emitting Ca(2+) probes and discuss their utility for biological Ca(2+) imaging. Using the spectral separability index Xijk (Oheim M., 2010. Methods in Molecular Biology 591: 3-16) we evaluate their performance for multi-color excitation/emission experiments, involving the identification of morphological landmarks with GFP/YFP and detecting Ca(2+)-dependent fluorescence in the red spectral band. We also establish a catalog of criteria for evaluating Ca(2+) indicators that ideally should be made available for each probe. This article is part of a Special Issue entitled: Calcium signaling in health and disease. Guest Editors: Geert Bultynck, Jacques Haiech, Claus W. Heizmann, Joachim Krebs, and Marc Moreau. |
| اللغة: | English |
| تدمد: | 0006-3002 |
| DOI: | 10.1016/j.bbamcr.2014.03.010⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::206e1c45dadaa7e010dab9fcb7ca2e12 https://hal.archives-ouvertes.fr/hal-00969335 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....206e1c45dadaa7e010dab9fcb7ca2e12 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00063002 |
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| DOI: | 10.1016/j.bbamcr.2014.03.010⟩ |