A 588-gene microarray analysis of the peripheral blood mononuclear cells of spondyloarthropathy patients
| العنوان: | A 588-gene microarray analysis of the peripheral blood mononuclear cells of spondyloarthropathy patients |
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| المؤلفون: | Eric Veys, Feng Huang, J G Kuipers, Wen Chan Tsai, D.L. Baeten, Jieruo Gu, Momiao Xiong, J. Kalsi, Dafna D. Gladman, Elisabeth Märker-Hermann, Leslie J. Crofford, Yeong Wook Song, Walter P. Maksymowych, H. Deister, Markus Rihl, D T Y Yu, F De Keyser, M. Bannai, N. Seta |
| المساهمون: | Clinical Immunology and Rheumatology |
| المصدر: | Rheumatology (Oxford, England), 41(7), 759-766. Oxford University Press Scopus-Elsevier |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Adult, Genetic Markers, Male, CCR1, Receptors, CXCR4, Adolescent, Spondyloarthropathy, Arthritis, Peripheral blood mononuclear cell, Arthritis, Rheumatoid, Psoriatic arthritis, Rheumatology, medicine, Humans, Spondylitis, Ankylosing, Pharmacology (medical), Aged, Oligonucleotide Array Sequence Analysis, Reverse Transcriptase Polymerase Chain Reaction, business.industry, Janus kinase 3, Arthritis, Psoriatic, Synovial Membrane, MNDA, Interleukin, DNA, Middle Aged, medicine.disease, Antigens, Differentiation, Chemokine CXCL12, Immunology, Leukocytes, Mononuclear, Female, business, Chemokines, CXC |
| الوصف: | OBJECTIVES: To identify genes which are more highly expressed in the peripheral blood mononuclear cells (PBMC) of patients with spondyloarthropathy (SpA), rheumatoid arthritis (RA) and psoriatic arthritis (PsA), in comparison to normal subjects. METHODS: A 588-gene microarray was used as a screening tool to select a panel of such genes from PBMC of these subjects and of normal subjects. Results were then validated by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: The following genes were more highly expressed in arthritis patients than in normal subjects: macrophage differentiation marker MNDA (myeloid nuclear differentiation antigen), MRP8 and MRP14 (migratory inhibitory factor-related proteins); signalling molecules JAK3 (janus kinase 3) and MAP kinase p38 (mitogen-activated protein kinase); receptors TNFR2/p75, C-C-chemokine receptor type 1 (CCR1), C-X-C-chemokine receptor type 4 (CXCR4) and integrin beta1; and the cytokines/chemokines interleukin (IL) 1beta and IL-8. Expression of CXCR4 was unexpectedly high among all arthritis subjects. Using RT-PCR, ELISA and immunohistology, expression of stromal cell-derived factor 1 (SDF-1) was demonstrated in arthritis joints. CONCLUSIONS: The CXCR4/SDF-1 is a potential pro-inflammatory axis for RA, PsA and SpA |
| اللغة: | English |
| تدمد: | 1462-0324 |
| DOI: | 10.1093/rheumatology/41.7.759 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1fc6283aa0c660799ca4408d46b0bb0e https://doi.org/10.1093/rheumatology/41.7.759 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1fc6283aa0c660799ca4408d46b0bb0e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14620324 |
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| DOI: | 10.1093/rheumatology/41.7.759 |