Structure-based design of a B cell antigen from B. pseudomallei
| العنوان: | Structure-based design of a B cell antigen from B. pseudomallei |
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| المؤلفون: | Giorgio Colombo, Davide Gaudesi, Giovanna Musco, Richard W. Titball, Alessandro Gori, Arnone Nithichanon, Oscar Conchillo-Solé, Mario Ferrer-Navarro, Rachael J. Thomas, Giacomo Quilici, Xavier Daura, Ganjana Lertmemongkolchai, Claudio Peri |
| المصدر: | ACS chemical biology (Online) 10 (2015): 803–812. doi:10.1021/cb500831y info:cnr-pdr/source/autori:Gaudesi D.; Peri C.; Quilici G.; Gori A.; Ferrer-Navarro M.; Conchillo-Sole O.; Thomas R.; Nithichanon A.; Lertmemongkolchai G.; Titball R.; Daura X.; Colombo G.; Musco G./titolo:Structure-based design of a B cell antigen from B. pseudomallei/doi:10.1021%2Fcb500831y/rivista:ACS chemical biology (Online)/anno:2015/pagina_da:803/pagina_a:812/intervallo_pagine:803–812/volume:10 |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Melioidosis, Burkholderia pseudomallei, Molecular Sequence Data, Molecular Dynamics Simulation, Pre-B-Cells, Protein Engineering, Biochemistry, Epitope, Protein Structure, Secondary, Epitopes, Structure-Activity Relationship, B pseudomallei, Agglutination Tests, medicine, Animals, Humans, Amino Acid Sequence, Antigens, Bacterial, B-Lymphocytes, biology, business.industry, Immune Sera, General Medicine, biology.organism_classification, medicine.disease, Virology, Antibodies, Bacterial, Recombinant Proteins, Protein Structure, Tertiary, Epitope mapping, biology.protein, Molecular Medicine, Structure based, Rabbits, Antibody, business, Epitope Mapping |
| الوصف: | Burkholderia pseudomallei is the etiological agent of melioidosis, a severe endemic disease in South-East Asia, causing septicemia and organ failure with high mortality rates. Current treatments and diagnostic approaches are largely ineffective. The development of new diagnostic tools and vaccines toward effective therapeutic opportunities against B. pseudomallei is therefore an urgent priority. In the framework of a multidisciplinary project tackling melioidosis through reverse and structural vaccinology, BPSL1050 was identified as a candidate for immunodiagnostic and vaccine development based on its reactivity against the sera of melioidosis patients. We determined its NMR solution structure and dynamics, and by novel computational methods we predicted immunogenic epitopes that once synthesized were able to elicit the production of antibodies inducing the agglutination of the bacterium and recognizing both BPSL1050 and B. pseudomallei crude extracts. Overall, these results hold promise for novel chemical biology approaches in the discovery of new diagnostic and prophylactic tools against melioidosis. |
| تدمد: | 1554-8937 |
| DOI: | 10.1021/cb500831y |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1faef76f3939ee6390b9d4c0343f6b80 https://pubmed.ncbi.nlm.nih.gov/25495888 |
| رقم الانضمام: | edsair.doi.dedup.....1faef76f3939ee6390b9d4c0343f6b80 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15548937 |
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| DOI: | 10.1021/cb500831y |