التفاصيل البيبلوغرافية
| العنوان: |
519. Immune responses and COVID-19 severity |
| المؤلفون: |
Christopher D. Brown, Charles Kyriakos Vorkas, Mirella Salvatore, Giorgio Inghirami, Joseph Casano, Grant B Ellsworth, Shashi Kapatia, Ayana Morales, Rosemary Soave, Harjot Sing, Jingmei Hsu, Kotha Saito |
| المصدر: |
Open Forum Infectious Diseases |
| بيانات النشر: |
Oxford University Press (OUP), 2020. |
| سنة النشر: |
2020 |
| مصطلحات موضوعية: |
business.industry, medicine.medical_treatment, T cell, Respiratory disease, Disease, medicine.disease, AcademicSubjects/MED00290, Cytokine, medicine.anatomical_structure, Immune system, Infectious Diseases, Oncology, Poster Abstracts, Severity of illness, Immunology, Medicine, Cytotoxic T cell, business, CD8 |
| الوصف: |
Background The coronavirus-19-disease (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread to >200 countries and surpassed 7 million cases. There is a broad range of COVID-19 illness, ranging from milder disease to a rapidly progressive respiratory disease and ARDS. The causes of this different clinical course and the drivers for severe disease are currently unknown. A fulminant increase of pro-inflammatory cytokines is thought to play a role in causing a rapid disease evolution, however the immune correlates of severe COVID-19 remain unclear. Methods To gain insight into relationship between immune responses and disease severity we built a longitudinal cohort of 40 adult patients with known COVID-19. Samples were collected at diagnosis and every 7 days until hospital discharge or death. As controls we also included a group of convalescent patients, and subjects who tested negative for COVID-19 by PCR. Clinical and laboratory data and were also collected. Multicolor flow cytometry was used to determine the presence and phenotype of B, T and natural killer (NK) cells. We also identified specific sub-populations (Tfh, activated/cytotoxic CD8 and NK) and assessed lymphoid exhaustion of different cell types such as naïve, memory T cells, or NK over time. Anti-Sars-CoV2 IgG and IgM antibody were detected using lateral flow method. Results We found that the absolute number of lymphocytes and monocytes was decreased starting at diagnosis and correlated with disease severity. Disease severity correlated with decreased NK and T cell. In severe COVID-19 cases, NK cell populations were strongly decreased over time in intubated patients while they recovered in patients who improved and were discharged. CD8+ were also decreased at disease onset and seemed to correlate with disease severity. A high percentage of CD4+ and CD8+ T cells showed an exhausted phenotype. All patients tested at admission had IgM antibody responses irrespective of the course of the disease. Further analyses are ongoing. Conclusion The characterization and role of the immune responses in COVID-19 evolution is still under investigation. Further characterization of viral and immune factors will help in identifying subjects at high risk of severe disease and targets for intervention. Disclosures All Authors: No reported disclosures |
| اللغة: |
English |
| تدمد: |
2328-8957 |
| DOI: |
10.1093/ofid/ofaa439.713 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f45f77c5d8757301d7f2e8193fc7f49 |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....1f45f77c5d8757301d7f2e8193fc7f49 |
| قاعدة البيانات: |
OpenAIRE |