Cosmetic applications of glucitol-core containing gallotannins from a proprietary phenolic-enriched red maple (Acer rubrum) leaves extract: inhibition of melanogenesis via down-regulation of tyrosinase and melanogenic gene expression in B16F10 melanoma cells
| العنوان: | Cosmetic applications of glucitol-core containing gallotannins from a proprietary phenolic-enriched red maple (Acer rubrum) leaves extract: inhibition of melanogenesis via down-regulation of tyrosinase and melanogenic gene expression in B16F10 melanoma cells |
|---|---|
| المؤلفون: | Hang Ma, Nicholas A. DaSilva, Shelby L. Johnson, Wei Lu, Liangran Guo, Navindra P. Seeram, Jialin Xu, Qiong Gu, Jun Xu, Zhengxi Wei, Ling Wang |
| المصدر: | Archives of Dermatological Research. 309:265-274 |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Skin Neoplasms, Tyrosinase, Melanoma, Experimental, Acer, Dermatology, Melanin, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Phenols, Western blot, Gene expression, medicine, Animals, Humans, Sorbitol, Cell Proliferation, Melanins, chemistry.chemical_classification, Reactive oxygen species, Natural product, medicine.diagnostic_test, Plant Extracts, Skin whitening, General Medicine, Hydrolyzable Tannins, Gene Expression Regulation, Neoplastic, Intramolecular Oxidoreductases, Plant Leaves, 030104 developmental biology, Enzyme, chemistry, Biochemistry, 030220 oncology & carcinogenesis, Melanocytes |
| الوصف: | The red maple (Acer rubrum) is a rich source of phenolic compounds which possess galloyl groups attached to different positions of a 1,5-anhydro-d-glucitol core. While these glucitol-core containing gallotannins (GCGs) have reported anti-oxidant and anti-glycative effects, they have not yet been evaluated for their cosmetic applications. Herein, the anti-tyrosinase and anti-melanogenic effects of a proprietary phenolic-enriched red maple leaves extract [Maplifa™; contains ca. 45% ginnalin A (GA) along with other GCGs] were investigated using enzyme and cellular assays. The GCGs showed anti-tyrosinase activity with IC50 values ranging from 101.4 to 1047.3 μM and their mechanism of tyrosinase inhibition (using GA as a representative GCG) was evaluated by chelating and computational/modeling studies. GA reduced melanin content in murine melanoma B16F10 cells by 79.1 and 56.7% (at non-toxic concentrations of 25 and 50 μM, respectively), and its mechanisms of anti-melanogenic effects were evaluated by using methods including fluorescent probe (DCF-DA), real-time PCR, and western blot experiments. These data indicated that GA was able to: (1) reduce the levels of reactive oxygen species, (2) down-regulate the expression of MITF, TYR, TRP-1, and TRP-2 gene levels in a time-dependent manner, and (3) significantly reduce protein expression of the TRP-2 gene. Therefore, the anti-melanogenic effects of red maple GCGs warrant further investigation of this proprietary natural product extract for potential cosmetic applications. |
| تدمد: | 1432-069X 0340-3696 |
| DOI: | 10.1007/s00403-017-1728-1 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ed47b150247c83b5f30d35f594d5be4 https://doi.org/10.1007/s00403-017-1728-1 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....1ed47b150247c83b5f30d35f594d5be4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1432069X 03403696 |
|---|---|
| DOI: | 10.1007/s00403-017-1728-1 |