Comparative genomics of cultured and uncultured strains suggests genes essential for free-living growth of Liberibacter
| العنوان: | Comparative genomics of cultured and uncultured strains suggests genes essential for free-living growth of Liberibacter |
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| المؤلفون: | Eric W. Triplett, Christopher S. Henry, Michael Davis, Jennie R. Fagen, Michael T. Leonard, Janaka N. Edirisinghe, Connor M. McCullough |
| المصدر: | PLoS ONE PLoS ONE, Vol 9, Iss 1, p e84469 (2014) |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Stringent response, Gene Identification and Analysis, Plant Pathogens, lcsh:Medicine, Genomics, Crops, Plant Science, Biology, Genome, Microbiology, Bacterial genetics, Electron Transport, Molecular Genetics, Plant Microbiology, Species Specificity, Rhizobiaceae, Culture Techniques, Homeostasis, KEGG, lcsh:Science, Gene, Pathogen, Microbial Metabolism, Genetics, Comparative genomics, Regulatory Networks, Evolutionary Biology, Multidisciplinary, Genes, Essential, lcsh:R, Cell Membrane, Crop Diseases, Computational Biology, Agriculture, Comparative Genomics, Plant Pathology, Carbon, Organismal Evolution, Functional Genomics, Genes, Bacterial, Microbial Evolution, lcsh:Q, Sequence Analysis, Research Article |
| الوصف: | The full genomes of two uncultured plant pathogenic Liberibacter, Ca. Liberibacter asiaticus and Ca. Liberibacter solanacearum, are publicly available. Recently, the larger genome of a closely related cultured strain, Liberibacter crescens BT-1, was described. To gain insights into our current inability to culture most Liberibacter, a comparative genomics analysis was done based on the RAST, KEGG, and manual annotations of these three organisms. In addition, pathogenicity genes were examined in all three bacteria. Key deficiencies were identified in Ca. L. asiaticus and Ca. L. solanacearum that might suggest why these organisms have not yet been cultured. Over 100 genes involved in amino acid and vitamin synthesis were annotated exclusively in L. crescens BT-1. However, none of these deficiencies are limiting in the rich media used to date. Other genes exclusive to L. crescens BT-1 include those involved in cell division, the stringent response regulatory pathway, and multiple two component regulatory systems. These results indicate that L. crescens is capable of growth under a much wider range of conditions than the uncultured Liberibacter strains. No outstanding differences were noted in pathogenicity-associated systems, suggesting that L. crescens BT-1 may be a plant pathogen on an as yet unidentified host. |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ea51566460b8c2fb1a2cc33eea2cd1f https://pubmed.ncbi.nlm.nih.gov/24416233 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1ea51566460b8c2fb1a2cc33eea2cd1f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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