التفاصيل البيبلوغرافية
| العنوان: |
Characterization of a microfluidic dispensing system for localised stimulation of cellular networks |
| المؤلفون: |
Tobias Kraus, Andreas Hierlemann, Flavio Heer, Teruo Fujii, Sadik Hafizovic, W. Franks, Vincent Linder, Elisabeth Verpoorte, Nico F. de Rooij, Sander Koster |
| المساهمون: |
Medicinal Chemistry and Bioanalysis (MCB) |
| المصدر: |
Lab on a Chip, 6(2), 218-229. ROYAL SOC CHEMISTRY |
| سنة النشر: |
2006 |
| مصطلحات موضوعية: |
Engineering, Indoles, ELECTRODES, Microfluidics, Cell Culture Techniques, Biomedical Engineering, Bioengineering, Nanotechnology, MICROELECTRODE ARRAY, Biochemistry, Cell Line, FLOWS, Drug Delivery Systems, Animals, CULTURED NEURONS, Myocytes, Cardiac, business.industry, CHIP, General Chemistry, Multielectrode array, Microfluidic Analytical Techniques, Chip, Characterization (materials science), CMOS, Proof of concept, Drug delivery, CELLS, Cellular network, business, Algorithms |
| الوصف: |
We present a 3-D microfluidic device designed for localized drug delivery to cellular networks. The device features a flow cell comprising a main channel for nutrient delivery as well as multiple channels for drug delivery. This device is one key component of a larger, fully integrated system now under development, based upon a microelectrode array (MEA) with on-chip CMOS circuitry for recording and stimulation of electrogenic cells (e.g. neurons, cardiomyocytes). As a critical system unit, the microfluidics must be carefully designed and characterized to ensure that candidate drugs are delivered to specific regions of the culture at known concentrations. Furthermore, microfluidic design and functionality is dictated by the size, geometry, and material/electrical characteristics of the CMOS MEA. Therefore, this paper reports on the design considerations and fabrication of the flow cell, including theoretical and experimental analysis of the mass transfer properties of the nutrient and drug flows, which are in good agreement with one another. To demonstrate proof of concept, the flow cell was mounted on a dummy CMOS chip, which had been plated with HL-1 cardiomyocytes. A test chemical compound was delivered to the cell culture in a spatially resolved manner. Envisioned applications of this stand-alone system include simultaneous toxicological testing of multiple compounds and chemical stimulation of natural neural networks for neuroscience investigations. © The Royal Society of Chemistry 2006. |
| اللغة: |
English |
| تدمد: |
1473-0197 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ea2dd2625195fa86c2df208e048d0c8
https://hdl.handle.net/11370/378a4809-a710-4538-8fa4-366faf96cac7 |
| Rights: |
CLOSED |
| رقم الانضمام: |
edsair.doi.dedup.....1ea2dd2625195fa86c2df208e048d0c8 |
| قاعدة البيانات: |
OpenAIRE |