A versatile magnetic bead-based flow cytometric assay for the detection of thyroid cancer related hsa-miR-221-3p in blood and tissues
| العنوان: | A versatile magnetic bead-based flow cytometric assay for the detection of thyroid cancer related hsa-miR-221-3p in blood and tissues |
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| المؤلفون: | Zhenxin Wang, Yaoqi Wang, Jia Wei, Jiaxue Gao, Xianying Meng, Lina Ma, Zhenshengnan Li |
| المصدر: | The Analyst. 146:842-847 |
| بيانات النشر: | Royal Society of Chemistry (RSC), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Biochemistry, Analytical Chemistry, Thyroid carcinoma, chemistry.chemical_compound, Complementary DNA, microRNA, Electrochemistry, medicine, Humans, Environmental Chemistry, Thyroid Neoplasms, Thyroid cancer, Spectroscopy, Detection limit, Chemistry, Magnetic Phenomena, RNA, Flow Cytometry, 021001 nanoscience & nanotechnology, medicine.disease, Molecular biology, In vitro, 0104 chemical sciences, MicroRNAs, Thyroid Cancer, Papillary, 0210 nano-technology, DNA |
| الوصف: | In vitro detection of low abundance biomolecules including microRNAs (miRNAs) is essential to biological research and early clinical diagnosis. In this work, a versatile magnetic bead (MB)-based flow cytometric assay was developed for the detection of hsa-miR-221-3p, which is strongly associated with papillary thyroid carcinoma (PTC). In the presence of hsa-miR-221-3p, the complementary DNA probe attached to the surface of MBs is hybridized with the target to form DNA/RNA heteroduplexes. After the recognition of the DNA/RNA heteroduplexes by PicoGreen, the fluorescence signals of each MB were readily detected using a flow cytometer. This assay can selectively detect hsa-miR-221-3p with a detection limit of 2.1 pM. The practicality of the assay is demonstrated by the discrimination of thyroid cancer tissues from normal tissues, and a satisfactory result is obtained. Moreover, this assay can be rapidly carried out in one step at room temperature, providing a generic method for the sensitive detection of miRNAs in molecular diagnosis. |
| تدمد: | 1364-5528 0003-2654 |
| DOI: | 10.1039/d0an02074e |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1db93476fd37cc5002ef9a988f8e6b42 https://doi.org/10.1039/d0an02074e |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....1db93476fd37cc5002ef9a988f8e6b42 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13645528 00032654 |
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| DOI: | 10.1039/d0an02074e |