Expression analysis of LTR-derived miR-1269a and target gene, KSR2 in Sebastes schlegelii
| العنوان: | Expression analysis of LTR-derived miR-1269a and target gene, KSR2 in Sebastes schlegelii |
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| المؤلفون: | Jennifer Im, Hee-Eun Lee, Ahran Kim, Do-Hyung Kim, Woo Ryung Kim, Heui-Soo Kim, Hee-Jae Cha, Yung Hyun Choi, Suhkmann Kim |
| المصدر: | Genes & Genomics. 42:55-65 |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Fish Proteins, 0106 biological sciences, 0301 basic medicine, Transposable element, Retroelements, ved/biology.organism_classification_rank.species, Sequence Homology, Retrotransposon, Genome browser, Protein Serine-Threonine Kinases, Biology, 01 natural sciences, Biochemistry, 03 medical and health sciences, microRNA, Genetics, Animals, Enhancer, 3' Untranslated Regions, Molecular Biology, Gene, Base Sequence, ved/biology, Gene Expression Profiling, Terminal Repeat Sequences, Computational Biology, Human genetics, Perciformes, MicroRNAs, 030104 developmental biology, Gene Expression Regulation, Sebastes schlegelii, 010606 plant biology & botany |
| الوصف: | Sebastes schlegelii are an important species of fish found in the coastal areas of the Korea with significant commercial importance. Most studies thus far have been primarily focused on environmental factors; behavioural patterns, aquaculture, diseases and limited genetic studies with little to none related to either microRNAs (miRNAs) or transposable elements (TE). In order to understand biological roles of TE-derived miR-1269a, we examined expression pattern for miR-1269a and its target gene, KSR2, in various tissues of Sebastes schlegelii. Also, we performed luciferase reporter assay in HINAE cells. UCSC Genome Browser (https://genome.ucsc.edu/) was used to examine which TE is associated with miR-1269a. For the target genes for miR-1269a, the target genes associated with the miRNA were identified using miRDB (http://www.mirdb.org/) and TargetScan 7.1 (http://www.targetscan.org/vert_71/). A two-step miRNA kit, HB miR Multi Assay Kit™ System. I was used for the analysis of TE-derived miRNA expression patterns. The 3′UTR of KSR2 gene was cloned into the psiCHECK-2 vector. Subsequently co-transfected with miR-1269a mimics to HINAE cells for luciferase reporter assay. MiR-1269a was found to be derived from LTR retrotransposon, MLT2B. LTR-derived miR-1269a was highly expressed in the muscle, liver and gonad tissues of Sebastes schlegelii, but KSR2 revealed high expression in the brain. Co-transfection of KSR2 and miR-1269a mimic to HINAE cells showed high activity of miR-1269a in relation to KSR2. LTR-derived miR-1269a showed enhancer activity with relation to KSR2 in Sebastes schlegelii. The data may be used as a foundation for further investigation regarding correlation of miRNA and target genes in addition to other functional studies of biological significance in Sebastes schlegelii. |
| تدمد: | 2092-9293 1976-9571 |
| DOI: | 10.1007/s13258-019-00880-0 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d39901f5250b61384101e31f6511cdc https://doi.org/10.1007/s13258-019-00880-0 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....1d39901f5250b61384101e31f6511cdc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20929293 19769571 |
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| DOI: | 10.1007/s13258-019-00880-0 |