Changes in colonic contractility in response to inflammatory bowel disease: Long-term assessment in a model of TNBS-induced inflammation in rats
| العنوان: | Changes in colonic contractility in response to inflammatory bowel disease: Long-term assessment in a model of TNBS-induced inflammation in rats |
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| المؤلفون: | José Ricardo de Arruda Miranda, André G. Próspero, Fábio P. F. Mello, Marcos F.F. Calabresi, Alexandre Tanimoto, Guilherme Soares, Luiz Claudio Di Stasi |
| المساهمون: | Universidade Estadual Paulista (Unesp) |
| المصدر: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| بيانات النشر: | Elsevier B.V., 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, medicine.medical_specialty, Contraction (grammar), Motility, Inflammation, Colonic contractility, 030226 pharmacology & pharmacy, Inflammatory bowel disease, Gastroenterology, General Biochemistry, Genetics and Molecular Biology, Contractility, 03 medical and health sciences, Alternating current biosusceptometry, 0302 clinical medicine, Intestinal inflammation, Internal medicine, medicine, Animals, General Pharmacology, Toxicology and Pharmaceutics, Intestinal Mucosa, Rats, Wistar, Gastrointestinal tract, business.industry, General Medicine, medicine.disease, Colitis, Inflammatory Bowel Diseases, Rats, Disease Models, Animal, 030104 developmental biology, Trinitrobenzenesulfonic Acid, medicine.symptom, business, Colonic motility, Muscle Contraction |
| الوصف: | Made available in DSpace on 2020-12-10T19:39:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-11-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Aims: Inflammatory bowel disease is a chronic relapsing inflammation that affects the gastrointestinal tract, causing changes in colonic motility. The evolution of these changes is not completely understood and possibly related to symptoms that appear in different degrees of the intestinal inflammation. Therefore, our aim is evaluate during 14 days of assessment aspects of colonic contractility using 2,4,6-trinitrobenzenesulfonic acid (TNBS) model of inflammation in rats and associate the inflammatory process with colonic motility. Methods: Contractility and inflammatory parameters were assessed in the same animal in six different moments: before intestinal inflammation induction, 2, 5, 8, 11, and 14 days after induction. The mechanical activity was determined by alternating current biosusceptometry (ACB) and subdivided into rhythmic propagating ripples (RPR) and rhythmic propulsive motor complexes (RPMC). We assessed inflammation by determining myeloperoxidase activity in feces. Results: Transient and permanent changes were observed in colonic motility as a function of the inflammatory process evaluated through myeloperoxidase activity. We identified two contraction profiles: RPR and RPMC. The microscopic analysis demonstrated a depth of damage caused by an injury that was associated with changes in motility. Conclusions: We implemented a robust and adequate (specific) signal processing to quantify two measured colonic frequency patterns. Thus, we performed a detailed temporal analysis of the consequences of TNBS-induced inflammation on colonic motility in rats. Our approach enables further long-term assessments in the same animal with different mechanisms and duration of injury, remission, treatments and their motor consequences. Sao Paulo State Univ, Biosci Inst, Dept Phys & Biophys, Lab Biomagnetism, Botucatu, SP, Brazil Sao Paulo State Univ, Biosci Inst, Dept Pharmacol, Lab Phytomed Pharmacol & Biotechnol, Botucatu, SP, Brazil Sao Paulo State Univ, Biosci Inst, Dept Phys & Biophys, Lab Biomagnetism, Botucatu, SP, Brazil Sao Paulo State Univ, Biosci Inst, Dept Pharmacol, Lab Phytomed Pharmacol & Biotechnol, Botucatu, SP, Brazil FAPESP: 2013/09095-4 FAPESP: 2015/14923-9 |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c13de7adfd280901a5a383fc581a642 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1c13de7adfd280901a5a383fc581a642 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |